LOCUS       sequence-277822-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2030
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggccgc tgcggacggc ccggctgaga cccaaagccc ggtggaaaaa
       61 gacagcccgg cgaagaccca aagcccagcc caagacacct caatcatgtc gagaaataac
      121 gcagatacag gcagagttct tgccttacca gagcacaaga agaagcgcaa gggaaacttg
      181 ccagccgagt ccgttaagat cctccgcgac tggatgtata agcatcggtt taaggcctac
      241 ccttcagaag aagagaagca aatgctgtca gagaagacca atttgtcttt gttgcagatt
      301 tctaactggt ttatcaatgc tcgcagacgc attctcccgg atatgcttca acagcgtaga
      361 aacgacccca tcattggcca caaaacgggc aaagatgccc atgccaccca cctgcagagc
      421 accgaggcgt ctgtgccggc caagtcaggg cccagtggtc cagacaatgt acaaagcctg
      481 cccctgtggc ccttgccaaa gggccagatg tcaagagaga agcaaccaga tccggagtcg
      541 gcccctagcc agaagctcac cggaatagcc cagccgaaga aaaaggtcaa ggtttctgtc
      601 acatccccgt cttctccaga acttgtgtct ccagaggagc acgccgactt cagcagcttc
      661 ctgctgctag tcgatgcagc agtacaaagg gctgccgagc tggagctaga gaagaagcaa
      721 gagcctaatc catagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
      781 tatatatctt gtggaaagga cgatgtactc tagcatactc gtgccat
//
