LOCUS       sequence-27783-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     TRONO lab plasmids
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRRL_H1shmp53_PGK_eGFP_Teto
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             59..239
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    join(278..296,297..319,320..338,339..361,362..380,381..403,
                     404..422,423..445,446..464,465..487,488..506,507..529,
                     530..548)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             789..800
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cngacgcgcg tatacgactc actatagggc gaattgggta cgtccctagg ctagatgctg
       61 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
      121 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
      181 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
      241 gtacaagcaa aaagcagatc gatccgactt tcacttttct ctatcactga tagggagtgg
      301 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
      361 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      421 gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta aactcgactt
      481 tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact
      541 gatagggagt ggtaaactcg agatcttgtc ttcgttggga gtgaattagc ccttccagtc
      601 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
      661 aaggtacctg gccatacgta ccgatgcggg gagncggccc aaagggagat ccgactcgtc
      721 tgagggcgaa ggcgaagacg cggaagagcc gcagagccng cagcagccgc gtgaaggaag
      781 tccgctggat tgagggccga aggacgtagc a
//
