LOCUS       sequence-277846-        1919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2070
FEATURES             Location/Qualifiers
     source          1..1919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agacacagaa ccacacatgc agctaccccg ggtggcacag caccaccatt
      901 gccgaccaca ccagacctca tggagacagt gcacctgttt cctgtttggg agaacaccac
      961 tccactccat ctctgccagc ggatcctggc tccctacctg aagaaagcgc ctcgccagca
     1021 aggtgcatga tcgtccacca gggcaccatt ctggataatg ttaagaacct cttagaattt
     1081 gcagaaacac tccaatttat agattctttc ttaaacactt ccagtaacca tgaaaactca
     1141 gacttggaaa tgccttcttt aacttccacc cccctcattg gtcacaaatt gactgttaca
     1201 acaccatttc atagagacca gactgtgaaa actcaaaagg aaaatactgt ttttagaacc
     1261 ccagctatca aaaggtcaat cttagaaagc tctccaagaa ctcctacacc attcaaacat
     1321 gcacttgcag ctcaagaaat taaatacggt cccctgaaga tgctacctca gacaccctct
     1381 catctagtag aagatctgca ggatgtgatc aaacaggaat ctgatgaatc tggaattgtt
     1441 gctgagtttc aagaaaatgg accaccctta ctgaagaaaa tcaaacaaga ggtggaatct
     1501 ccaactgata aatcaggaaa cttcttctgc tcacaccact gggaagggga cagtctgaat
     1561 acccaactgt tcacgcagac ctcgcctgtg gcagatgcac cgaatattct tacaagctcc
     1621 gttttaatgg caccagcatc agaagatgaa gacaatgttc tcaaagcatt tacagtacct
     1681 aaaaacaggt ccctggcgag ccccttgcag ccttgtagca gtacctggga acctgcatcc
     1741 tgtggaaaga tggaggagca gatgacatct tccagtcaag ctcgtaaata cgtgaatgca
     1801 ttctcagccc ggacgctggt catgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1861 atttcttggc tttatatatc ttgtggaaag gacgagctcc cactcctcga tggtcgcaa
//
