LOCUS       sequence-277852-        2024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2072
FEATURES             Location/Qualifiers
     source          1..2024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcaggt gctaccaaca
      961 cagaaccaca catgcagcta ccccgggtgg cacagcacca ccattgccga ccacaccaga
     1021 cctcatggag acagtgcacc tgtttcctgt ttgggagaac accactccac tccatctctg
     1081 ccagcggatc ctggctccct acctgaagaa agcgcctcgc cagcaaggtg catgatcgtc
     1141 caccagggca ccattctgga taatgttaag aacctcttag aatttgcaga aacactccaa
     1201 tttatagatt ctttcttaaa cacttccagt aaccatgaaa actcagactt ggaaatgcct
     1261 tctttaactt ccacccccct cattggtcac aaattgactg ttacaacacc atttcataga
     1321 gaccagactg tgaaaactca aaaggaaaat actgttttta gaaccccagc tatcaaaagg
     1381 tcaatcttag aaagctctcc aagaactcct acaccattca aacatgcact tgcagctcaa
     1441 gaaattaaat acggtcccct gaagatgcta cctcagacac cctctcatct agtagaagat
     1501 ctgcaggatg tgatcaaaca ggaatctgat gaatctggaa ttgttgctga gtttcaagaa
     1561 aatggaccac ccttactgaa gaaaatcaaa caagaggtgg aatctccaac tgataaatca
     1621 ggaaacttct tctgctcaca ccactgggaa ggggacagtc tgaataccca actgttcacg
     1681 cagacctcgc ctgtggcaga tgcaccgaat attcttacaa gctccgtttt aatggcacca
     1741 gcatcagaag atgaagacaa tgttctcaaa gcatttacag tacctaaaaa caggtccctg
     1801 gcgagcccct tgcagccttg tagcagtacc tgggaacctg catcctgtgg aaagatggag
     1861 gagcagatga catcttccag tcaagctcgt aaatacgtga atgcattctc agcccggacg
     1921 ctggtcatgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1981 atatcttgtg gaaaggacga cataaactcc ctattggcct ctct
//
