LOCUS       sequence-277855-        2378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2073
FEATURES             Location/Qualifiers
     source          1..2378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcagac acagaaccac
      961 acatgcagct accccgggtg gcacagcacc accattgccg accacaccag acctcatgga
     1021 gacagtgcac ctgtttcctg tttgggagaa caccactcca ctccatctct gccagcggat
     1081 cctggctccc tacctgaaga aagcgcctcg ccagcaaggt gcatgatcgt ccaccagggc
     1141 accattctgg ataatgttaa gaacctctta gaatttgcag aaacactcca atttatagat
     1201 tctgattctt catcatggtg tgatctcagc agttttgaat tctttgaaga agcagatttt
     1261 tcacctagcc aacatcacac aggcaaagcc ctacagcttc agcaaagaga gggcaatggg
     1321 actaaacctg caggagaacc tagcccaagg gtgaacaaac gtatgttgag tgagagttca
     1381 cttgacccac ccaaggtctt acctcctgca aggcacagca caattccact ggtcatcctt
     1441 cgaaaaaaac ggggccaggc cagcccctta gccactggag actgtagctc cttcatattt
     1501 gctgacgtca gcagttcaac tcccaagcgt tcccctgtca aaagcctacc cttctctccc
     1561 tcgcagttct taaacacttc cagtaaccat gaaaactcag acttggaaat gccttcttta
     1621 acttccaccc ccctcattgg tcacaaattg actgttacaa caccatttca tagagaccag
     1681 actgtgaaaa ctcaaaagga aaatactgtt tttagaaccc cagctatcaa aaggtcaatc
     1741 ttagaaagct ctccaagaac tcctacacca ttcaaacatg cacttgcagc tcaagaaatt
     1801 aaatacggtc ccctgaagat gctacctcag acaccctctc atctagtaga agatctgcag
     1861 gatgtgatca aacaggaatc tgatgaatct ggaattgttg ctgagtttca agaaaatgga
     1921 ccacccttac tgaagaaaat caaacaagag gtggaatctc caactgataa atcaggaaac
     1981 ttcttctgct cacaccactg ggaaggggac agtctgaata cccaactgtt cacgcagacc
     2041 tcgcctgtgg cagatgcacc gaatattctt acaagctccg ttttaatggc accagcatca
     2101 gaagatgaag acaatgttct caaagcattt acagtaccta aaaacaggtc cctggcgagc
     2161 cccttgcagc cttgtagcag tacctgggaa cctgcatcct gtggaaagat ggaggagcag
     2221 atgacatctt ccagtcaagc tcgtaaatac gtgaatgcat tctcagcccg gacgctggtc
     2281 atgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2341 tgtggaaagg acgagaatga cctgtgttgt ggtcgtgg
//
