LOCUS       sequence-277858-        2339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2074
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcaggt gctaccaaca
      961 cagaaccaca catgcagcta ccccgggtgg cacagcacca ccattgccga ccacaccaga
     1021 cctcatggag acagtgcacc tgtttcctgt ttgggagaac accactccac tccatctctg
     1081 ccagcggatc ctggctccct acctgaagaa agcgcctcgc cagcaaggtg catgatcgtc
     1141 caccagggca ccattctgga taatgattct tcatcatggt gtgatctcag cagttttgaa
     1201 ttctttgaag aagcagattt ttcacctagc caacatcaca caggcaaagc cctacagctt
     1261 cagcaaagag agggcaatgg gactaaacct gcaggagaac ctagcccaag ggtgaacaaa
     1321 cgtatgttga gtgagagttc acttgaccca cccaaggtct tacctcctgc aaggcacagc
     1381 acaattccac tggtcatcct tcgaaaaaaa cggggccagg ccagcccctt agccactgga
     1441 gactgtagct ccttcatatt tgctgacgtc agcagttcaa ctcccaagcg ttcccctgtc
     1501 aaaagcctac ccttctctcc ctcgcagttc ttaaacactt ccagtaacca tgaaaactca
     1561 gacttggaaa tgccttcttt aacttccacc cccctcattg gtcacaaatt gactgttaca
     1621 acaccatttc atagagacca gactgtgaaa actcaaaagg aaaatactgt ttttagaacc
     1681 ccagctatca aaaggtcaat cttagaaagc tctccaagaa ctcctacacc attcaaacat
     1741 gcacttgcag ctcaagaaat taaatacggt cccctgaaga tgctacctca gacaccctct
     1801 catctagtag aagatctgca ggatgtgatc aaacaggaat ctgatgaatc tggaattgtt
     1861 gctgagtttc aagaaaatgg accaccctta ctgaagaaaa tcaaacaaga ggtggaatct
     1921 ccaactgata aatcaggaaa cttcttctgc tcacaccact gggaagggga cagtctgaat
     1981 acccaactgt tcacgcagac ctcgcctgtg gcagatgcac cgaatattct tacaagctcc
     2041 gttttaatgg caccagcatc agaagatgaa gacaatgttc tcaaagcatt tacagtacct
     2101 aaaaacaggt ccctggcgag ccccttgcag ccttgtagca gtacctggga acctgcatcc
     2161 tgtggaaaga tggaggagca gatgacatct tccagtcaag ctcgtaaata cgtgaatgca
     2221 ttctcagccc ggacgctggt catgtagtaa tgaactagtc cgtaacttga aagtatttcg
     2281 atttcttggc tttatatatc ttgtggaaag gacgagtgga cttccagtat cgttagtaa
//
