LOCUS       sequence-277861-        2387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2075
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcaggt gctaccaaca
      961 cagaaccaca catgcagcta ccccgggtgg cacagcacca ccattgccga ccacaccaga
     1021 cctcatggag acagtgcacc tgtttcctgt ttgggagaac accactccac tccatctctg
     1081 ccagcggatc ctggctccct acctgaagaa agcgcctcgc cagcaaggtg catgatcgtc
     1141 caccagggca ccattctgga taatgttaag aacctcttag aatttgcaga aacactccaa
     1201 tttatagatt ctgattcttc atcatggtgt gatctcagca gttttgaatt ctttgaagaa
     1261 gcagattttt cacctagcca acatcacaca ggcaaagccc tacagcttca gcaaagagag
     1321 ggcaatggga ctaaacctgc aggagaacct agcccaaggg tgaacaaacg tatgttgagt
     1381 gagagttcac ttgacccacc caaggtctta cctcctgcaa ggcacagcac aattccactg
     1441 gtcatccttc gaaaaaaacg gggccaggcc agccccttag ccactggaga ctgtagctcc
     1501 ttcatatttg ctgacgtcag cagttcaact cccaagcgtt cccctgtcaa aagcctaccc
     1561 ttctctccct cgcagttctt aaacacttcc agtaaccatg aaaactcaga cttggaaatg
     1621 ccttctttaa cttccacccc cctcattggt cacaaattga ctgttacaac accatttcat
     1681 agagaccaga ctgtgaaaac tcaaaaggaa aatactgttt ttagaacccc agctatcaaa
     1741 aggtcaatct tagaaagctc tccaagaact cctacaccat tcaaacatgc acttgcagct
     1801 caagaaatta aatacggtcc cctgaagatg ctacctcaga caccctctca tctagtagaa
     1861 gatctgcagg atgtgatcaa acaggaatct gatgaatctg gaattgttgc tgagtttcaa
     1921 gaaaatggac cacccttact gaagaaaatc aaacaagagg tggaatctcc aactgataaa
     1981 tcaggaaact tcttctgctc acaccactgg gaaggggaca gtctgaatac ccaactgttc
     2041 acgcagacct cgcctgtggc agatgcaccg aatattctta caagctccgt tttaatggca
     2101 ccagcatcag aagatgaaga caatgttctc aaagcattta cagtacctaa aaacaggtcc
     2161 ctggcgagcc ccttgcagcc ttgtagcagt acctgggaac ctgcatcctg tggaaagatg
     2221 gaggagcaga tgacatcttc cagtcaagct cgtaaatacg tgaatgcatt ctcagcccgg
     2281 acgctggtca tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2341 tatatatctt gtggaaagga cgatgctgat aagggccgac cctggcc
//
