LOCUS       sequence-277864-        2015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2077
FEATURES             Location/Qualifiers
     source          1..2015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgaag accccggcac agcatatata gcagtgacga ggatgatgag
       61 gactttgaga tgtgtgacca tgactatgat gggctgcttc ccaagtctgg aaagcgtcac
      121 ttggggaaaa caaggtggac ccgggaagag gatgaaaaac tgaagaagct ggtggaacag
      181 aatggaacag atgactggaa agttattgcc aattatctcc cgaatcgaac agatgtgcag
      241 tgccagcacc gatggcagaa agtactaaac cctgagctca tcaagggtcc ttggaccaaa
      301 gaagaagatc agagagtgat agagcttgta cagaaatacg gtccgaaacg ttggtctgtt
      361 attgccaagc acttaaaggg gagaattgga aaacaatgta gggagaggtg gcataaccac
      421 ttgaatccag aagttaagaa aacctcctgg acagaagagg aagacagaat tatttaccag
      481 gcacacaaga gactggggaa cagatgggca gaaatcgcaa agctactgcc tggacgaact
      541 gataatgcta tcaagaacca ctggaattct acaatgcgtc ggaaggtcga acaggaaggt
      601 tatctgcagg agtcttcaaa agccagccag ccagcagtgg ccacaagctt ccagaagaac
      661 agtcatttga tgggttttgc tcaggctccg cctacagctc aactccctgc cactggccag
      721 cccactgtta acaacgacta ttcctattac cacatttctg aagcacaaaa tgtctccagt
      781 catgttccat accctgtagc gttacatgta aatatagtca atgtccctca gccagctgcc
      841 gcagccattc agagacacta taatgatgaa gaccctgaga aggaaaagcg aataaaggaa
      901 ttagaattgc tcctaatgtc aaccgagaat gagctaaaag gacagcagac acagaaccac
      961 acatgcagct accccgggtg gcacagcacc accattgccg accacaccag acctcatgga
     1021 gacagtgcac ctgtttcctg tttgggagaa caccactcca ctccatctct gccagcggat
     1081 cctggctccc tacctgaaga aagcgcctcg ccagcaaggt gcatgatcgt ccaccagggc
     1141 accattctgg ataatgttaa gaacctctta gaatttgcag aaacactcca atttatagat
     1201 tctttcttaa acacttccag taaccatgaa aactcagact tggaaatgcc ttctttaact
     1261 tccacccccc tcattggtca caaattgact gttacaacac catttcatag agaccagact
     1321 gtgaaaactc aaaaggaaaa tactgttttt agaaccccag ctatcaaaag gtcaatctta
     1381 gaaagctctc caagaactcc tacaccattc aaacatgcac ttgcagctca agaaattaaa
     1441 tacggtcccc tgaagatgct acctcagaca ccctctcatc tagtagaaga tctgcaggat
     1501 gtgatcaaac aggaatctga tgaatctgga attgttgctg agtttcaaga aaatggacca
     1561 cccttactga agaaaatcaa acaagaggtg gaatctccaa ctgataaatc aggaaacttc
     1621 ttctgctcac accactggga aggggacagt ctgaataccc aactgttcac gcagacctcg
     1681 cctgtggcag atgcaccgaa tattcttaca agctccgttt taatggcacc agcatcagaa
     1741 gatgaagaca atgttctcaa agcatttaca gtacctaaaa acaggtccct ggcgagcccc
     1801 ttgcagcctt gtagcagtac ctgggaacct gcatcctgtg gaaagatgga ggagcagatg
     1861 acatcttcca gtcaagctcg taaatacgtg aatgcattct cagcccggac gctggtcatg
     1921 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1981 ggaaaggacg aaaggcacgg gtcggcctct tcgtt
//
