LOCUS       sequence-277873-        1262 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2080
FEATURES             Location/Qualifiers
     source          1..1262
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagggagg actggcaggc gagcgggcga gggagtctcc ggtggactgc
       61 agcgttagca aatgcagcaa gctggtgggc ggaggcgagt ccaaccccat gaactacaac
      121 agctatatgg acgagaagaa tggcccccct cctcccaaca tgaccaccaa cgagaggaga
      181 gtcatcgtcc ccgcagaccc cacactgtgg acacaggagc atgtgaggca atggctggag
      241 tgggccataa aggagtacag cttgatggag atcgacacat cctttttcca gaacatggat
      301 ggcaaggaac tgtgtaaaat gaacaaggag gacttcctcc gcgccaccac cctctacaac
      361 acggaagtgc tgttgtcaca cctcagttac ctcagggaaa gttcactgct ggcctataat
      421 acaacctccc acaccgacca atcctcacga ttgagtgtca aagaagaccc ttcttatgac
      481 tcagtcagaa gaggagcttg gggcaataac atgaattctg gcctcaacaa aagtcctccc
      541 cttggagggg cacaaacgat cagtaagaat acagagcaac ggccccagcc agatccgtat
      601 cagatcctgg gcccgaccag cagtcgccta gccaaccctg gaagcgggca gatccagctg
      661 tggcaattcc tcctggagct gctctccgac agcgccaacg ccagctgtat cacctgggag
      721 gggaccaacg gggagttcaa aatgacggac cccgatgagg tggccaggcg ctggggcgag
      781 cggaaaagca agcccaacat gaattacgac aagctgagcc gggccctccg ttattactat
      841 gataaaaaca ttatgaccaa agtgcacggc aaaagatatg cttacaaatt tgacttccac
      901 ggcattgccc aggctctgca gccacatccg accgagtcgt ccatgtacaa gtacccttct
      961 gacatctcct acatgccttc ctaccatgcc caccagcaga aggtgaactt tgtccctccc
     1021 catccatcct ccatgcctgt cacttcctcc agcttctttg gagccgcatc acaatactgg
     1081 acctccccca cggggggaat ctaccccaac cccaacgtcc cccgccatcc taacacccac
     1141 gtgccttcac acttaggcag ctactactag taatgaacta gtccgtaact tgaaagtatt
     1201 tcgatttctt ggctttatat atcttgtgga aaggacgaag catcaacgcg cccgctagac
     1261 gg
//
