LOCUS       sequence-277876-        3083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2082
FEATURES             Location/Qualifiers
     source          1..3083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2251..2262)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accgccacca tggcctcggc agctgtggag agcttcgtga ccaagcaact ggacctgctg
       61 gagcttgaga gagacgcgga ggtggaggag cgcaggtcct ggcaggagaa catctctctg
      121 aaagagctcc agagccgagg cgtgtgtttg ctgaagctgc aggtatccag ccagcgcact
      181 gggctgtacg gacggctgct ggtcaccttt gagcccaggc gatacgggtc cgcggcagct
      241 cttcccagta acagctttac ttctggtgat atcgtgggcc tgtacgatgc tgctaatgag
      301 ggcagtcagc tggccactgg gatcttgacc cgggtcaccc agaagtcggt cacggtggcc
      361 tttgatgagt cccacgattt ccagttgagc ttggaccgag agaattccta cagactgtta
      421 aaacttgcca atgatgtcac ttacaggcga ctgaaaaaag ccctgattgc tctaaagaag
      481 tatcattctg gcccagcctc ctcactcata gaagtgctct ttggcagatc tgctcccagt
      541 cctgccagtg aaatacaccc gctgacattc ttcaacacct gcctggacac ctcccagaaa
      601 gaagcggttt tatttgcgct gtctcagaaa gaacttgcca tcatccatgg acctcctggc
      661 actgggaaaa ccacgactgt ggttgagatc attcttcaag ctgtgaaaca aggcttaaag
      721 gttctgtgct gcgccccctc caacatcgcc gtggacaatc tggtggagcg cctggctctg
      781 tgtaagcagc ggattctgcg cctgggacac cctgcccgcc tcctggagtc cattcagcag
      841 cactccctgg atgcggtttt agcgcggagc gacagtgccc agattgttgc agatatcagg
      901 aaggacatcg accaggtctt tgtgaaaaac aaaaagaccc aggataagag agagaaaagt
      961 aattttcgaa atgaaattaa gctgttaaga aaagaactga aggagaggga agaagcagct
     1021 atgctcgaga gcctcacttc ggcaaacgtg gtccttgcaa caaacacagg tgcgtctgcc
     1081 gatggccccc tgaagttgct gcccgagagc tacttcgacg tggtggtcat tgacgagtgt
     1141 gcccaggccc tcgaggcgag ctgctggatc cccctgctga aggccagaaa gtgcatcctg
     1201 gcgggcgatc acaagcagct gccccccacc acagtctctc acaaggctgc gctggcagga
     1261 ctgtcactca gcctgatgga acgcctggct gaggagtacg gcgcgagggt ggtgcggaca
     1321 ctgacggtgc agtaccgcat gcaccaggct atcatgcgct gggcctcaga caccatgtac
     1381 cttgggcagc tcacagccca ctcttccgtg gcaaggcacc tcctgaggga cctcccaggt
     1441 gtggctgcca cagaagagac gggtgtgccc ctgctcttgg tggacaccgc cggctgcggg
     1501 ctgtttgagc tggaggagga ggacgaacag tcgaaaggga accctggcga agtccgcctc
     1561 gtcagtttgc acatccaggc tctggtggac gctggtgttc cagcccgtga cattgctgtg
     1621 gtctcgccat acaacctcca ggtggacctg ctcagacaga gccttgtgca caggcaccct
     1681 gagcttgaaa tcaagtctgt cgatggcttc caaggccgag agaaggaggc cgtgatactg
     1741 tccttcgtca gatccaacag gaaaggtgaa gttggttttc ttgctgagga ccggaggatc
     1801 aacgtggctg tcacccgtgc ccgacgccac gtggcggtca tctgtgactc ccgtactgtc
     1861 aacaaccatg catttttgaa gaccctggtg gagtatttca cacagcatgg ggaagtacgc
     1921 acggcctttg agtatcttga cgatattgtc ccagaaaact attcccatga gaactcccag
     1981 ggttccagcc acgctgccac caagccccag ggacctgcta cgtccaccag gaccggaagc
     2041 cagcggcagg agggaggcca ggaggctgca gcacctgcca gacagggccg gaagaagccg
     2101 gctgggaagt ctctggcctc tgaagctcca tctcagccca gcctcaacgg aggcagccca
     2161 gagggagtgg agagccaaga tggcgtggac cacttccggg ccatgatagt ggagttcatg
     2221 gccagcaaga agatgcagtt ggagtttcct ccttccctca attcccacga caggctgcgg
     2281 gtccaccaaa tagccgagga gcacgggctg aggcacgaca gttccgggga agggaagagg
     2341 aggttcatca ctgtgagcaa gagggccccg cgaccccgag cagccctggg acccccagca
     2401 gggaccggtg gcccagcccc tctccagcca gtgcccccta cccctgcgca gacagagcag
     2461 cctcccaggg agcagcgtgg cccagaccag cctgatctga ggacgctgca cctggagaga
     2521 ctgcagaggg tcaggagcgc gcaggggcag cccgccagca aggagcagca ggcctcaggg
     2581 cagcagaaac ttccagaaaa gaaaaagaaa aaagccaaag gacatccggc cacagatctg
     2641 cccacggagg aggactttga ggccctggtt tctgccgccg ttaaggctga taacacctgc
     2701 ggctttgcca agtgcacagc cggcgtcaca accctgggcc agttctgcca gctctgcagc
     2761 cgccgctact gcctcagcca ccacctgccc gagatccatg gctgcggtga gagggctcgc
     2821 gcccatgccc ggcagagaat cagccgggaa ggggtcctct atgccggcag cgggaccaag
     2881 aacggatccc tggacccagc caagagggcc cagctgcaga ggaggctgga taagaagctg
     2941 agtgagctca gcaaccagag gaccagccgg aggaaggaga gggggacgta gtaatgaact
     3001 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     3061 aatgtatatc ttaatagtat ggt
//
