LOCUS       sequence-277891-        3245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2095
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgcaga ggctgaagat aaaacgctgc gtactcgctc taaaggaacc
       61 gaggtgccaa tggattcact aatccaggag ctcagtgttg cctatgattg ctccatggca
      121 aagaagagaa cagctgaaga tcaggctttg ggggttccag tcaacaaaag gaaatccctg
      181 ctaatgaagc cccgacacta cagcccaaaa gcagactgcc aagaagaccg cagtgacagg
      241 acagaggacg atggcccctt ggaaacacat ggtcactcta ccgcagagga aatcatgata
      301 aaacctatgg atgaaagtct tctttcaact gcacaagaaa actccagtag gaaggaagac
      361 agatactctt gttatcaaga gctcatggtc aagtctttaa tgcacttggg gaaatttgaa
      421 aaaaatgtat ctgttcagac tgtaagtgaa aatttaaatg acagtggcat ccagtcttta
      481 aaagcagaga gcgatgaagc agacgagtgc tttctgattc attctgatga tggaagagac
      541 aagattgatg attctcagcc acccttctgc tcctctgatg acaatgaaag taactctgaa
      601 agtgcagaaa atggctggga cagtggctcc aacttctcag aagaaaccaa accacctaga
      661 gtcccaaagt atgttttaac agatcataaa aaagacctat tggaagttcc tgaaataaaa
      721 actgaaggtg acaaatttat cccttgtgag aacaggtgtg attctgaaac agaaaggaaa
      781 gacccgcaga atgctctcgc agaacccctg gatggcaatg cccagccctc attccctgac
      841 gttgaggagg aagatagcga gagcctggca gtaatgacgg aagagggtag tgacctggaa
      901 aaggccaagg ggaatttaag tttgctggag caggcaattg ctctgcaggc tgagcgaggt
      961 tgtgttttcc ataacaccta caaagagctg gataggttcc tgctggagca cctagcaggg
     1021 gaaaggaggc aaaccaaagt tatcgacatg ggtggaagac aaatctttaa caataaacat
     1081 tcaccaaggc ctgaaaagag ggagaccaag tgcccgatcc ctggatgtga tggcacggga
     1141 cacgtgacag ggctctaccc gcaccaccgc agcctttcgg ggtgccccca caaagtgcgg
     1201 gttcccctgg aaattcttgc catgcatgaa aatgtgctca agtgtcccac gccgggatgc
     1261 acaggaaggg gtcatgtgaa cagcaaccgc aacacccaca ggagtctttc tggttgtcca
     1321 attgctgcag ctgaaaaatt ggcaatgtcc caggataaaa atcagcttga ttctccccaa
     1381 actgggcagt gtcctgacca ggcccacagg acaagtttgg tgaagcaaat tgaattcaat
     1441 ttcccgtcac aagccatcac ctctcccaga gccacagtgt caaaagaaca agagaagttt
     1501 ggaaaagtac catttgatta tgccagtttt gatgcccaag ttttcggtaa acgccctctc
     1561 atacaaacag tgcaaggacg aaaaacacca ccatttcctg aatcaaagca ttttccaaat
     1621 ccagtgaaat ttcctaatcg actgcctagt gcaggcgccc acacccagag ccctggccgt
     1681 gccagctctt atagctacgg tcaatgtagt gaagacaccc acatagcagc agctgctgcc
     1741 atcctgaacc tttccacccg ctgcagggaa gccacagaca tcctctccaa caagccacag
     1801 agtctgcatg ccaagggagc cgaaatagaa gtggatgaaa atggcacatt ggacttaagc
     1861 atgaaaaaaa atcgaatcct ggacaagtct gcacccctaa cttcctctaa cacttctatt
     1921 ccaactcctt cctcttcccc attcaaaaca agcagcattc tggtcaatgc agcattctat
     1981 caggctcttt gtgaccaaga gggctgggac actcctatca actatagcaa aactcacggg
     2041 aagacagagg aggagaaaga gaaagaccca gtgagctctc tagaaaattt agaggaaaaa
     2101 aagtttcctg gagaggcctc tataccaagc cctaaaccca agcttcatgc aagagatctc
     2161 aaaaaggaac taatcacctg tccaacacca ggatgtgatg gaagtggcca cgtgacagga
     2221 aactatgcat ctcatcgcag tgtttctgga tgtcctttag cagataagac tctaaaatcc
     2281 ctcatggctg ccaactctca ggagcttaag tgtccaaccc caggctgcga tggctcgggg
     2341 cacgtgactg gaaactatgc ttcccacaga agcttgtccg gatgccctcg tgcaaggaaa
     2401 ggtggtgtca aaatgacccc taccaaggaa gaaaaagaag accctgaact gaaatgtcct
     2461 gtgatagggt gtgatggcca aggtcacata tcaggtaaat acacatcaca ccgcacagct
     2521 tctggctgtc ctctggctgc caagagacag aaggagaatc ctctcaatgg agcctccctc
     2581 tcctggaaac tgaacaaaca agagctacca cattgtccct tgccaggctg caatgggctg
     2641 ggccatgtaa ataatgtttt tgtcacccac cgaagcttat ctggatgtcc tctcaatgca
     2701 caagttatca aaaagggcaa ggtttctgaa gaactcatga ccatcaagct caaagcaact
     2761 gggggaatag agagtgatga agaaattagg catttggatg aagaaataaa ggaactgaat
     2821 gaatccaacc ttaaaattga agcagatatg atgaaacttc agacccagat cacatctatg
     2881 gagagcaact taaagacgat agaggaggag aacaaactca tagaacagaa caatgaaagt
     2941 ctgctgaaag agctggcagg tctaagccaa gctctcattt caagccttgc tgacatccag
     3001 cttccacaga tgggacctat cagtgagcag aattttgaag catatgtaaa tacactcaca
     3061 gatatgtaca gcaatctgga acgggactat tccccggaat gcaaagctct actggaaagt
     3121 atcaaacagg cagtgaaggg tatccatgtg tagtaatgaa ctagtccgta acttgaaagt
     3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggtacgtct attacatctg
     3241 ttgag
//
