LOCUS       sequence-277894-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2096
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcatctgc actattcaca gcggattccc aggcaatggt gaagatcgag
       61 gacatggctg tgtccctcat tctggaggaa tggggatgtc agaatctggc tcggaggaat
      121 ctcagtaggg acaacaggca ggagaattat gggagcgcat ttccccaggg tggtgaaaac
      181 aggaatgaga acgaggagtc aacctcaaag gctgaaacct cggaagattc agcatcacgc
      241 ggggagacaa caggaagatc ccagaaagag tttggagaga aacgtgacca ggagggcaaa
      301 acaggagaaa gacagcagaa aaaccctgag gagaaaacca ggaaagagaa aagagattca
      361 gggccagcta taggaaagga caaaaaaacc atcacaggag agagaggtcc aagggagaag
      421 gggaaaggat tgggaagaag cttcagtctg agctccaact tcaccacccc tgaagaagtt
      481 cccacgggaa caaagtctca cagatgtgat gaatgtggta aatgcttcac gagaagttca
      541 agccttatcc gccataaaat aatccacact ggagaaaagc cctatgaatg tagtgagtgt
      601 gggaaagcct tcagtcttaa ctccaacctt gtcctgcatc agaggatcca cacaggagag
      661 aaacctcatg aatgtaacga gtgtggcaag gccttcagcc acagttccaa tctcatcctc
      721 catcagcgca tccactctgg agagaaacct tatgaatgta atgagtgcgg gaaggccttc
      781 agccagagct cggacctcac caagcatcag agaattcaca cgggggagaa accctatgaa
      841 tgtagtgaat gtggaaaagc tttcaaccga aactcatacc tgattttgca tcggagaatt
      901 cacactcgag aaaagcccta caagtgcact aagtgtggca aggccttcac ccgcagctcc
      961 accctcactc tgcatcacag aatccatgcc agagagagag cctctgagta cagcccagcc
     1021 tcccttgatg catttggcgc gttcctgaaa agttgtgtgt agtaatgaac tagtccgtaa
     1081 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga atgaccggag
     1141 gttagaagcc atga
//
