LOCUS       sequence-277903-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2100
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcctc aattatctgc caggagcact gccagatctc gggccaggca
       61 aaaatagaca tcttattagt tggagatgtc actgtgggct acctggctga tactgtacag
      121 aaactatttg caaacatagc agaagtcacc atcaccatca gtgacacgaa ggaggcagca
      181 gcgcttttgg atgattgcat attcaacatg gttctcttga aggtgccttc ttcactaagt
      241 gccgaggagc tggaagccat caagttaatt agatttggca aaaagaaaaa tacacattca
      301 ctgtttgttt ttataatccc tgaaaatttt aaaggttgta tttcagggca tggaatggat
      361 attgctttaa ctgaaccact gacaatggaa aaaatgagta atgtggtaaa atactggaca
      421 acatgtccct caaacactgt taagactgaa aacgcaactg ggcctgaaga acttggattg
      481 cccctgcaga ggtcctacag cgaacacctg ggatattttc ctactgatct atttgcctgc
      541 tctgaatctt taaggaatgg caatgggctt gaattaaatg cttcgttgtc agagttcgag
      601 aaaaacaaaa agatctctct tcttcattca agcaaggaaa aactaagaag gctgtacagg
      661 aagcatagca gcttctgttt ctggtagtaa tgaactagtc cgtaacttga aagtatttcg
      721 atttcttggc tttatatatc ttgtggaaag gacgagcgac gacggttaca tcgtgatat
//
