LOCUS       sequence-27791-e         352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 (dominant-negative mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(44..153)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    169..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(254..273)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gaaccgtgat ggtggtgatg gtgtgccatg gtatatctcc tttgattgta aataaaatgt
       61 aatttacagt atagtatttt aattaatata caaatgattt gataataatt cttatttaac
      121 tataatatat tgtgttgggt tgaattaaag gtcccggatt aactccgggg aattgttatc
      181 cgctcacaat tcccctatag tgagtcgtat taatttcggt ccgatccaat tctttgccaa
      241 aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg
      301 catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag cc
//
