LOCUS       sequence-277924-         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2118
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgcaga cagtgatgtt gcattggaca ttctaattac aaatgtagtc
       61 tgtgttttta gaacaagatg tcatttaaac ttaaggaaga ttgctttgga aggagcaaat
      121 gtaatttata aacgtgatgt tggaaaagta ttaatgaagc ttagaaaacc tagaattaca
      181 gctacaattt ggtcctcagg aaaaattatt tgcactggag caacaagtga agaagaagct
      241 aaatttggtg ccagacgctt agcccgtagt ctgcagaaac taggttttca ggtaatattt
      301 acagatttta aggttgttaa cgttctggca gtgtgtaaca tgccatttga aatccgtttg
      361 ccagaattca caaagaacaa tagacctcat gccagttacg aacctgaact tcatcctgct
      421 gtgtgctatc ggataaaatc tctaagagct acattacaga ttttttcaac aggaagtatc
      481 acagtaacag ggcccaatgt aaaggctgtt gctactgctg tggaacagat ttacccattt
      541 gtgtttgaaa gcaggaaaga aattttatag taatgaacta gtccgtaact tgaaagtatt
      601 tcgatttctt ggctttatat atcttgtgga aaggacgaat acttgtgtga ggttagcagc
      661 tc
//
