LOCUS       sequence-27793-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(237..665)
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
     primer_bind     complement(726..747)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaagcaatta agaacacatc tgtttgcgga taggataggg ggcgtaatct gtcataatct
       61 tcttgtccag ctgtatccca taagcccaga ttcaccggtt ttccatctac cataacattg
      121 gcagaataat tgtcaaagac agtagggata tattctccag gaaatgcatt ggttgtgtaa
      181 ctgatcagta ggcaattttt acctacagct ccgtctccca ccaccacaca cttgataagt
      241 tcttttgccg cctcatcaat attttctgca gttttcttaa tcagcatgac tccctctctc
      301 tcggcagcat ctcggacatg ctcagttcca tccaactgaa ccccaataaa gtactggaca
      361 tctcccttct gatctcgcat aggctgcaag tgaaagaggt tccagaactt tttaccactc
      421 tttgtataat taatcagctg aacagtgacc tctgtttggt tatctatggc atctctaatt
      481 tttctcactg tcgcgcgatc agtttcagga ccttgtagaa acctgcagtt tcttcccaaa
      541 atttcttcac ggctatattc tgtcaactgc aagaaactat cggacgcgaa tataatggga
      601 ttatctggca atcttgggtc agtaatgaca aagttcttct caatacgttc aagtgtagta
      661 gccaaggatc cacttccaga acccttgtac agctcgtcca tgccgagagt gatcccggcg
      721 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagt
      781 ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      841 gtgttctgct ggtagtggtc ggcgagct
//
