LOCUS       sequence-277933-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2122
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgcagt ggcctttgag gatgtggctg tgaacttcac ccaggaggaa
       61 tgggctttgc tgggtccatc acagaagaat ttatacagat atgtgatgca agaaaccatc
      121 aggaacctgg actgtataag aatgatatgg gaagaacaga atactgaaga tcagtacaaa
      181 aatcctagga gaaatctaag atgtcatatg gtagagagat tcagtgaaag taaagacagt
      241 agtcagtgtg gagaaacatt tagcctcatt cgagatagta ttgtgaacaa cagcatttgt
      301 cctggagaag atccatgtca aagcgctgag tgtgaagaag tcataatggg tcatttatcc
      361 cttaatagcc acatcagagt tgattctgga cacaaaccac atgagtatca ggaatatgga
      421 gagaagccac atacacataa acaacgtggg aaagccttca gttaccatca ctcctttcag
      481 tcgcgtggaa ggcctcacac tggaaagaaa cgctatgagt gtaaggaatg tggaaaaacc
      541 ttcagttctc gtagaaacct tcgaagacac atggtagtgc aaggtggaaa tagaccttat
      601 aaatttccta tcgaagacat gagagaatgc acactgggga gaaaccgtat gaatgtaagc
      661 agtgttctaa agccttgcct gattccagtt cctatatagt aatgaactag tccgtaactt
      721 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagta tatgggttgt
      781 tgccaataat a
//
