LOCUS       sequence-27796-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Rac1 fused with light-insensitive LOV2 (C450A 
            mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1-C450A
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             630..662
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             669..692
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ttgatgaggc ggcnaaagaa cttatcaagt gtgtggtggt gggagacgga gctgtaggta
       61 aaacttgcct actgatcagt tacacaacca atgcatttcc tggagaatat atccctactg
      121 tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat ctgggcttat
      181 gggatacagc tggactagaa gattatgaca gattacgccc cctatcctat ccgcaaacag
      241 atgtgttctt aatttgcttt tcccttgtga gtcctgcatc attccaccac gtccgtgcaa
      301 agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta gtgggaacta
      361 aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag aagctgactc
      421 ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta aaatacctgg
      481 agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg atccgagcag
      541 tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg taaaagcttg
      601 cggccgcaca gctgtataca cgtgcaagcc agccagaact cgccccggaa gaccccgagg
      661 atctcgagca ccaccatcac catcaccatc actaagtgat taacctcagg tgcaggctgc
      721 ctatcaaaag ggggggc
//
