LOCUS       sequence-277960-        1793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2145
FEATURES             Location/Qualifiers
     source          1..1793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaactc aagtttcatt ggggatcatg tccatggagc tcctcatgct
       61 gtcatgccag aagtggaatt ccctgaccaa ttctttacag ttctaaccat ggaccatgaa
      121 ctggtgacac tcagggatgt ggtcatcaac ttctctcagg aggaatggga atatctggat
      181 tctgctcaga ggaacttgta ctgggatgtg atgatggaga actacagcaa cttactctca
      241 ctggatttgg agtccaggaa tgagactaag catttatctg taggaaaaga tattattcaa
      301 aacactggtt ctcagtggga ggtaatggaa agtagcaagt tatgtggcct tgaaggttcc
      361 attttcagaa atgactggca aagcaaaagc aagattgact tacaaggacc tgaagtggga
      421 tatttcagtc aaatgaaaat catctctgaa aatgtgccca gttacaaaac tcatgaatct
      481 cttactttac ctcggagaac tcatgacagt gagaagccct atgaatacaa ggaatatgag
      541 aaggtcttca gttgtgactt agagtttgat gaatatcaga aaatacatac tggtggaaaa
      601 aactatgaat gtaatcaatg ttggaaaacc tttgggatag ataactccag tatgttacaa
      661 ctgaatattc atactggtgt gaaaccttgt aaatatatgg aatatgggaa tacatgtagt
      721 ttttataaag actttaatgt ataccagaaa attcataatg agaagttcta taaatgtaag
      781 gaatacagaa ggacctttga aagagttgga aaagttactc cacttcaaag agttcatgat
      841 ggtgagaaac actttgaatg ctcattctgt gggaaatcct ttagagtgca tgcacaactt
      901 actcgacatc agaaaatcca tactgatgag aaaacttaca aatgtatgga atgtggcaag
      961 gacttcagat ttcattcaca gcttaccgaa catcagagaa ttcatactgg tgagaaaccc
     1021 tacaaatgta tgcactgtga gaaggttttt agaattagtt cacagctcat tgaacatcag
     1081 agaattcaca ctggtgagaa accttatgca tgtaaggaat gtgggaaggc ttttggagta
     1141 tgtagagaac ttgctcgtca tcagagaatt catactggta agaaacccta tgaatgcaaa
     1201 gcatgtggaa aggtctttag aaatagttca tccctgacta gacatcagag gattcatact
     1261 ggtgaaaaac cctataaatg taaagaatgt gagaaagcat ttggagtagg tagtgaactt
     1321 actcgacatg aaagaattca cagtggtcaa aaaccttatg aatgtaagga gtgtggaaag
     1381 ttctttagac ttacgtcagc ccttattcaa catcaaagaa ttcatagtgg tgagaaacct
     1441 tatgaatgta aggtatgtgg gaaggccttt agacatagtt cagcccttac agaacatcag
     1501 agaattcata ctggagaaaa accctatgaa tgcaaggcat gtgggaaggc ctttagacat
     1561 agttcatcct ttaccaaaca tcagcgcatt catactgatg ataaacccta tgaatgtaag
     1621 gagtgtggga attcctttag tgttgttggg catcttactt gccaaccgaa aatttacact
     1681 ggtgagaaat catttgacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1741 tggctttata tatcttgtgg aaaggacgaa cattacgtga tcggtagata gcg
//
