LOCUS       sequence-277963-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2146
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaagtag caagttatgt ggccttgaag gttccatttt cagaaatgac
       61 tggcaaagca aaagcaagat tgacttacaa ggacctgaag tgggatattt cagtcaaatg
      121 aaaatcatct ctgaaaatgt gcccagttac aaaactcatg aatctcttac tttacctcgg
      181 agaactcatg acagtgagaa gccctatgaa tacaaggaat atgagaaggt cttcagttgt
      241 gacttagagt ttgatgaata tcagaaaata catactggtg gaaaaaacta tgaatgtaat
      301 caatgttgga aaacctttgg gatagataac tccagtatgt tacaactgaa tattcatact
      361 ggtgtgaaac cttgtaaata tatggaatat gggaatacat gtagttttta taaagacttt
      421 aatgtatacc agaaaattca taatgagaag ttctataaat gtaaggaata cagaaggacc
      481 tttgaaagag ttggaaaagt tactccactt caaagagttc atgatggtga gaaacacttt
      541 gaatgctcat tctgtgggaa atcctttaga gtgcatgcac aacttactcg acatcagaaa
      601 atccatactg atgagaaaac ttacaaatgt atggaatgtg gcaaggactt cagatttcat
      661 tcacagctta ccgaacatca gagaattcat actggtgaga aaccctacaa atgtatgcac
      721 tgtgagaagg tttttagaat tagttcacag ctcattgaac atcagagaat tcacactggt
      781 gagaaacctt atgcatgtaa ggaatgtggg aaggcttttg gagtatgtag agaacttgct
      841 cgtcatcaga gaattcatac tggtaagaaa ccctatgaat gcaaagcatg tggaaaggtc
      901 tttagaaata gttcatccct gactagacat cagaggattc atactggtga aaaaccctat
      961 aaatgtaaag aatgtgagaa agcatttgga gtaggtagtg aacttactcg acatgaaaga
     1021 attcacagtg gtcaaaaacc ttatgaatgt aaggagtgtg gaaagttctt tagacttacg
     1081 tcagccctta ttcaacatca aagaattcat agtggtgaga aaccttatga atgtaaggta
     1141 tgtgggaagg cctttagaca tagttcagcc cttacagaac atcagagaat tcatactgga
     1201 gaaaaaccct atgaatgcaa ggcatgtggg aaggccttta gacatagttc atcctttacc
     1261 aaacatcagc gcattcatac tgatgataaa ccctatgaat gtaaggagtg tgggaattcc
     1321 tttagtgttg ttgggcatct tacttgccaa ccgaaaattt acactggtga gaaatcattt
     1381 gactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgacagcct ttcctccaac tatgactg
//
