LOCUS       sequence-277966-        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2149
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcagc cagactcctg ccagtgccgg caggacccca ggccaagctg
       61 accttcgagg atgtggctgt gctcctctcc caggatgaat gggaccgcct gtgccctgct
      121 cagaggggtc tctacagaaa tgtgatgatg gaaacctatg ggaatgtagt ctcattggga
      181 cttccaggat ccaagcctga cataatctcc cagctggagc gaggggaaga tccctgggtc
      241 ctggacagga agggggctaa gaagagccag ggcctgtgga gtgactactc agacaacctc
      301 aaatatgacc acactacagc ctgtacacaa caagacagtt tatcttgtcc atgggaatgt
      361 gaaaccaagg gagagagtca aaatacagac ttgagtccga agccattaat ttcagagcaa
      421 acagtgattc tggggaaaac acccttgggg aggattgatc aagaaaataa tgaaacaaag
      481 caaagcttct gtctgagtcc aaactctgtt gaccaccgtg aagttcaggt cttaagccaa
      541 agcatgccac tcactccgca ccaggcagtg cctagtggag agaggcccta catgtgtgtt
      601 gagtgtggga agtgctttgg ccggagttcc cacctccttc agcatcagcg tatccacact
      661 ggagagaagc cctatgtgtg cagtgtatgt gggaaggcct tcagccagag ctcagtcctt
      721 agtaaacaca ggagaattca cacaggtgag aagccctatg agtgtaatga gtgtggaaaa
      781 gcctttagag tgagctcaga tcttgctcag catcacaaga tacatacagg agagaagcct
      841 cacgaatgtc ttgagtgtcg gaaagccttc actcaactct cacatctcat tcagcaccag
      901 cggatccaca cgggagaaag gccatatgtg tgtccgttgt gtgggaaagc cttcaaccat
      961 agcactgttc tgcggagcca ccagagggta cacactgggg agaagcctca caggtgcaat
     1021 gagtgtggga aaaccttcag tgtgaagagg acactgctgc agcaccagag gatccacacc
     1081 ggggagaagc cctacacgtg cagcgagtgt gggaaggcct tcagcgaccg ctcagtcctc
     1141 attcagcacc acaacgtgca caccggggag aagccctatg agtgcagtga gtgtgggaag
     1201 accttcagcc accgctccac actgatgaat cacgagcgga tccacaccga ggaaaagccc
     1261 tatgcatgct acgaatgtgg gaaggccttc gttcagcact cacacctgat ccagcaccag
     1321 agagtccaca ctggggagaa gccctatgtg tgtggtgaat gtgggcacgc cttcagtgca
     1381 cgccggtctc tgatccagca tgagagaatc cacacaggtg aaaagccctt ccagtgcaca
     1441 gaatgtggca aagccttcag cctgaaagca actctgattg tgcacctgag gacccacacg
     1501 ggcgagaagc catatgagtg caatagctgc gggaaggcct tcagccagta ctcagtgctc
     1561 atccagcacc agcggatcca cacaggcgag aagccctatg agtgcgggga gtgtgggcgt
     1621 gccttcaacc agcatggcca cctaatccag caccagaaag tgcacagaaa gttgtagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgagaaat agtgattgat ccctaggcg
//
