LOCUS       sequence-277969-        1784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2150
FEATURES             Location/Qualifiers
     source          1..1784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagcagc cagactcctg ccagtgccgg caggacccca gcccctgtcg
       61 ttccaggcca agctgacctt cgaggatgtg gctgtgctcc tctcccagga tgaatgggac
      121 cgcctgtgcc ctgctcagag gggtctctac agaaatgtga tgatggaaac ctatgggaat
      181 gtagtctcat tgggacttcc aggatccaag cctgacataa tctcccagct ggagcgaggg
      241 gaagatccct gggtcctgga caggaagggg gctaagaaga gccagggcct gtggagtgac
      301 tactcagaca acctcaaata tgaccacact acagcctgta cacaacaaga cagtttatct
      361 tgtccatggg aatgtgaaac caagggagag agtcaaaata cagacttgag tccgaagcca
      421 ttaatttcag agcaaacagt gattctgggg aaaacaccct tggggaggat tgatcaagaa
      481 aataatgaaa caaagcaaag cttctgtctg agtccaaact ctgttgacca ccgtgaagtt
      541 caggtcttaa gccaaagcat gccactcact ccgcaccagg cagtgcctag tggagagagg
      601 ccctacatgt gtgttgagtg tgggaagtgc tttggccgga gttcccacct ccttcagcat
      661 cagcgtatcc acactggaga gaagccctat gtgtgcagtg tatgtgggaa ggccttcagc
      721 cagagctcag tccttagtaa acacaggaga attcacacag gtgagaagcc ctatgagtgt
      781 aatgagtgtg gaaaagcctt tagagtgagc tcagatcttg ctcagcatca caagatacat
      841 acaggagaga agcctcacga atgtcttgag tgtcggaaag ccttcactca actctcacat
      901 ctcattcagc accagcggat ccacacggga gaaaggccat atgtgtgtcc gttgtgtggg
      961 aaagccttca accatagcac tgttctgcgg agccaccaga gggtacacac tggggagaag
     1021 cctcacaggt gcaatgagtg tgggaaaacc ttcagtgtga agaggacact gctgcagcac
     1081 cagaggatcc acaccgggga gaagccctac acgtgcagcg agtgtgggaa ggccttcagc
     1141 gaccgctcag tcctcattca gcaccacaac gtgcacaccg gggagaagcc ctatgagtgc
     1201 agtgagtgtg ggaagacctt cagccaccgc tccacactga tgaatcacga gcggatccac
     1261 accgaggaaa agccctatgc atgctacgaa tgtgggaagg ccttcgttca gcactcacac
     1321 ctgatccagc accagagagt ccacactggg gagaagccct atgtgtgtgg tgaatgtggg
     1381 cacgccttca gtgcacgccg gtctctgatc cagcatgaga gaatccacac aggtgaaaag
     1441 cccttccagt gcacagaatg tggcaaagcc ttcagcctga aagcaactct gattgtgcac
     1501 ctgaggaccc acacgggcga gaagccatat gagtgcaata gctgcgggaa ggccttcagc
     1561 cagtactcag tgctcatcca gcaccagcgg atccacacag gcgagaagcc ctatgagtgc
     1621 ggggagtgtg ggcgtgcctt caaccagcat ggccacctaa tccagcacca gaaagtgcac
     1681 agaaagttgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1741 atatcttgtg gaaaggacga tcttccacgg gccgctaata gcct
//
