LOCUS       sequence-27797-e         428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Rac1 fused with light-insensitive LOV2 (C450A 
            mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1-C450A
FEATURES             Location/Qualifiers
     source          1..428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(64..173)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    189..213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(213..232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(214..232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(274..293)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(355..377)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcaca gaaccgtgat ggtggtgatg gtgtgccatg gtatatctcc
       61 tttgattgta aataaaatgt aatttacagt atagtatttt aattaatata caaatgattt
      121 gataataatt cttatttaac tataatatat tgtgttgggt tgaattaaag gtcccggatt
      181 aactccgggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcggt
      241 ccgatccaat tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag
      301 ctgtaggaaa aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac
      361 acgccagaag ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg
      421 acagcccc
//
