LOCUS       sequence-277972-        2423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2152
FEATURES             Location/Qualifiers
     source          1..2423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..127
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgtctcgccg caagcaaggc aaaccccagc acttaagcaa acgggaattc
       61 tcgcccgagc ctcttgaagc cattcttaca gatgatgaac cagaccacgg cccgttggga
      121 gctccagaag gggatcatga cctcctcacc tgtgggcagt gccagatgaa cttcccattg
      181 ggggacattc ttatttttat cgagcacaaa cggaaacaat gcaatggcag cctctgctta
      241 gaaaaagctg tggataagcc accttcccct tcaccaatcg agatgaaaaa agcatccaat
      301 cccgtggagg ttggcatcca ggtcacgcca gaggatgacg attgtttatc aacgtcatct
      361 agaggaattt gccccaaaca ggaacacata gcagataaac ttctgcactg gaggggcctc
      421 tcctcccctc gttctgcaca tggagctcta atccccacgc ctgggatgag tgcagaatat
      481 gccccgcagg gtatttgtaa agatgagccc agcagctaca catgtacaac ttgcaaacag
      541 ccattcacca gtgcatggtt tctcttgcaa cacgcacaga acactcatgg attaagaatc
      601 tacttagaaa gcgaacacgg aagtcccctg accccgcggg ttggtatccc ttcaggacta
      661 ggtgcagaat gtccttccca gccacctctc catgggattc atattgcaga caataacccc
      721 tttaacctgc taagaatacc aggatcagta tcgagagagg cttccggcct ggcagaaggg
      781 cgctttccac ccactccccc cctgtttagt ccaccaccga gacatcactt ggacccccac
      841 cgcatagagc gcctgggggc ggaagagatg gccctggcca cccatcaccc gagtgccttt
      901 gacagggtgc tgcggttgaa tccaatggct atggagcctc ccgccatgga tttctctagg
      961 agacttagag agctggcagg gaacacgtct agcccaccgc tgtccccagg ccggcccagc
     1021 cctatgcaaa ggttactgca accattccag ccaggtagca agccgccctt cctggcgacg
     1081 ccccccctcc ctcctctgca atccgcccct cctccctccc agcccccggt caagtccaag
     1141 tcatgcgagt tctgcggcaa gacgttcaaa tttcagagca acctggtggt gcaccggcgc
     1201 agccacacgg gcgagaagcc ctacaagtgc aacctgtgcg accacgcgtg cacccaggcc
     1261 agcaagctga agcgccacat gaagacgcac atgcacaaat cgtcccccat gacggtcaag
     1321 tccgacgacg gtctctccac cgccagctcc ccggaacccg gcaccagcga cttggtgggc
     1381 agcgccagca gcgcgctcaa gtccgtggtg gccaagttca agagcgagaa cgaccccaac
     1441 ctgatcccgg agaacgggga cgaggaggaa gaggaggacg acgaggaaga ggaagaagag
     1501 gaggaagagg aggaggagga gctgacggag agcgagaggg tggactacgg cttcgggctg
     1561 agcctggagg cggcgcgcca ccacgagaac agctcgcggg gcgcggtcgt gggcgtgggc
     1621 gacgagagcc gcgccctgcc cgacgtcatg cagggcatgg tgctcagctc catgcagcac
     1681 ttcagcgagg ccttccacca ggtcctgggc gagaagcata agcgcggcca cctggccgag
     1741 gccgagggcc acagggacac ttgcgacgaa gactcggtgg ccggcgagtc ggaccgcata
     1801 gacgatggca ctgttaatgg ccgcggctgc tccccgggcg agtcggcctc ggggggcctg
     1861 tccaaaaagc tgctgctggg cagccccagc tcgctgagcc ccttctctaa gcgcatcaag
     1921 ctcgagaagg agttcgacct gcccccggcc gcgatgccca acacggagaa cgtgtactcg
     1981 cagtggctcg ccggctacgc ggcctccagg cagctcaaag atcccttcct tagcttcgga
     2041 gactccagac aatcgccttt tgcctcctcg tcggagcact cctcggagaa cgggagtttg
     2101 cgcttctcca caccgcccgg ggagctggac ggagggatct cggggcgcag cggcacggga
     2161 agtggaggga gcacgcccca tattagtggt ccgggcccgg gcaggcccag ctcaaaagag
     2221 ggcagacgca gcgacacttg ttcttcacac acccccattc ggcgtagtac ccagagagct
     2281 caagatgtgt ggcagttttc ggatggaagc tcgagagccc ttaagttcta gtaatgaact
     2341 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     2401 ttaccctacg gcgggcgaac tgg
//
