LOCUS       sequence-277975-        2507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2153
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..127
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgtctcgccg caagcaaggc aaaccccagc acttaagcaa acgggaattc
       61 tcgcccgagc ctcttgaagc cattcttaca gatgatgaac cagaccacgg cccgttggga
      121 gctccagaag gggatcatga cctcctcacc tgtgggcagt gccagatgaa cttcccattg
      181 ggggacattc ttatttttat cgagcacaaa cggaaacaat gcaatggcag cctctgctta
      241 gaaaaagctg tggataagcc accttcccct tcaccaatcg agatgaaaaa agcatccaat
      301 cccgtggagg ttggcatcca ggtcacgcca gaggatgacg attgtttatc aacgtcatct
      361 agaggaattt gccccaaaca ggaacacata gcaggtaaag atgagcccag cagctacaca
      421 tgtacaactt gcaaacagcc attcaccagt gcatggtttc tcttgcaaca cgcacagaac
      481 actcatggat taagaatcta cttagaaagc gaacacggaa gtcccctgac cccgcgggtt
      541 ggtatccctt caggactagg tgcagaatgt ccttcccagc cacctctcca tgggattcat
      601 attgcagaca ataacccctt taacctgcta agaataccag gatcagtatc gagagaggct
      661 tccggcctgg cagaagggcg ctttccaccc actccccccc tgtttagtcc accaccgaga
      721 catcacttgg acccccaccg catagagcgc ctgggggcgg aagagatggc cctggccacc
      781 catcacccga gtgcctttga cagggtgctg cggttgaatc caatggctat ggagcctccc
      841 gccatggatt tctctaggag acttagagag ctggcaggga acacgtctag cccaccgctg
      901 tccccaggcc ggcccagccc tatgcaaagg ttactgcaac cattccagcc aggtagcaag
      961 ccgcccttcc tggcgacgcc ccccctccct cctctgcaat ccgcccctcc tccctcccag
     1021 cccccggtca agtccaagtc atgcgagttc tgcggcaaga cgttcaaatt tcagagcaac
     1081 ctggtggtgc accggcgcag ccacacgggc gagaagccct acaagtgcaa cctgtgcgac
     1141 cacgcgtgca cccaggccag caagctgaag cgccacatga agacgcacat gcacaaatcg
     1201 tcccccatga cggtcaagtc cgacgacggt ctctccaccg ccagctcccc ggaacccggc
     1261 accagcgact tggtgggcag cgccagcagc gcgctcaagt ccgtggtggc caagttcaag
     1321 agcgagaacg accccaacct gatcccggag aacggggacg aggaggaaga ggaggacgac
     1381 gaggaagagg aagaagagga ggaagaggag gaggaggagc tgacggagag cgagagggtg
     1441 gactacggct tcgggctgag cctggaggcg gcgcgccacc acgagaacag ctcgcggggc
     1501 gcggtcgtgg gcgtgggcga cgagagccgc gccctgcccg acgtcatgca gggcatggtg
     1561 ctcagctcca tgcagcactt cagcgaggcc ttccaccagg tcctgggcga gaagcataag
     1621 cgcggccacc tggccgaggc cgagggccac agggacactt gcgacgaaga ctcggtggcc
     1681 ggcgagtcgg accgcataga cgatggcact gttaatggcc gcggctgctc cccgggcgag
     1741 tcggcctcgg ggggcctgtc caaaaagctg ctgctgggca gccccagctc gctgagcccc
     1801 ttctctaagc gcatcaagct cgagaaggag ttcgacctgc ccccggccgc gatgcccaac
     1861 acggagaacg tgtactcgca gtggctcgcc ggctacgcgg cctccaggca gctcaaagat
     1921 cccttcctta gcttcggaga ctccagacaa tcgccttttg cctcctcgtc ggagcactcc
     1981 tcggagaacg ggagtttgcg cttctccaca ccgcccgggg agctggacgg agggatctcg
     2041 gggcgcagcg gcacgggaag tggagggagc acgccccata ttagtggtcc gggcccgggc
     2101 aggcccagct caaaagaggg cagacgcagc gacacttgtg agtactgtgg gaaagtcttc
     2161 aagaactgta gcaatctcac tgtccacagg agaagccaca cgggcgaaag gccttataaa
     2221 tgcgagctgt gcaactatgc ctgtgcccag agtagcaagc tcaccaggca catgaaaacg
     2281 catggccagg tggggaagga cgtttacaaa tgtgaaattt gtaagatgcc ttttagcgtg
     2341 tacagtaccc tggagaaaca catgaaaaaa tggcacagtg atcgagtgtt gaataatgat
     2401 ataaaaactg aatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2461 tatatatctt gtggaaagga cgatcccagc tccggatcaa tagttat
//
