LOCUS       sequence-27798-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Rac1 fused with light-insensitive LOV2 (C450A 
            mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mVenus-PA-Rac1-C450A
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..464
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NARFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 tggacgagct gtacaagggt tctggaagtg gatccttggc tactacactt gaacgtattg
       61 agaagaactt tgtcattact gacccaagat tgccagataa tcccattata ttcgcgtccg
      121 atagtttctt gcagttgaca gaatatagcc gtgaagaaat tttgggaaga aacgcaaggt
      181 ttctacaagg tcctgaaact gatcgcgcga cagtgagaaa aattagagat gccatagata
      241 accaaacaga ggtcactgtt cagctgatta attatacaaa gagtggtaaa aagttctgga
      301 acctctttca cttgcagcct atgcgagatc agaagggaga tgtccagtac tttattgggg
      361 ttcagttgga tggaactgag catgtccgag atgctgccga gagagaggga gtcatgctga
      421 ttaagaaaac tgcagaaaat attgatgagg cggcaaaaga acttatcaag tgtgtggtgg
      481 tgggagacgg agctgtaggt aaaacttgcc tactgatcag ttacacaacc aatgcatttc
      541 ctggagaata tatccctact gtctttgaca attattctgc caatgttatg gtagatggaa
      601 aaccggtgaa tctgggctta tgggatacag ctggactaga agattatgac agattacgcc
      661 ccctatccta tccgcaaaca gatgtgttct taatttgctt ttcccttgtg agtcctgcat
      721 cattccaccn cgtccgtgca aagtggtatc ctgaggtgcg gcaccactgn tccc
//
