LOCUS       sequence-277990-        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2162
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcgct gtgggaatcc ccccagcagt gtatcatcct gagcccactg
       61 agcgggtggt ggttttcgat cggaatctca atactgacca gttcagctct ggtgctcaag
      121 ccccaaatgc tcaaaggcga actccagact cgaccttctc agagaccttc aaggaaggcg
      181 ttcaggagga acaactttga atatacccta gaagcttcaa aatcacttcg acagaagcca
      241 ggagacagta ccatgacgta cctgaacaaa ggccagttct atcccatcac cttgaaggag
      301 gtgagcagca gtgaaggaat ccatcatccc atcagcaaag ttcgaagtgt gatcatggtg
      361 gtttttgctg aagacaaaag cagagaagat cagttaaggc attggaagta ctggcactcc
      421 cggcagcaca ccgctaaaca aagatgcatt gacatagctg actataaaga aagcttcaac
      481 actatcagta acatcgagga gattgcgtat aacgccattt ccttcacatg ggacatcaac
      541 gatgaagcaa aggttttcat ctctgtgaac tgcttaagca cagatttctc ttcccagaag
      601 ggagtgaagg ggttgcctct taacattcaa gttgatacct atagttacaa caaccgcagc
      661 aacaagcctg tgcaccgggc ctactgccag atcaaggtct tctgtgacaa gggagctgag
      721 cggaaaatca gggatgaaga acgaaagcaa agcaaaagaa aagtttctga tgttaaagtg
      781 ccactgcttc cctctcacaa gcgaatggat atcacagttt tcaaaccctt cattgatctc
      841 gatactcagc ctgtcctctt cattcctgac gtgcactttg ccaacttgca gcggggcact
      901 catgtccttc ccattgcctc tgaagaattg gagggtgaag gctctgtctt gaaaaggggg
      961 ccgtacggca cagaagatga ctttgctgtc cctccttcta ccaagctggc ccggatagaa
     1021 gaaccaaaga gagtgctgct ctacgttcga aaggagtcag aagaagtctt tgatgccctg
     1081 atgctcaaaa ccccatcttt gaagggcttg atggaagcta tctcagacaa atacgatgtt
     1141 ccccatgaca agattgggaa aatattcaag aagtgtaaaa aggggatcct ggtgaacatg
     1201 gacgacaaca ttgtgaagca ttactccaat gaggacacct tccagctgca gattgaagaa
     1261 gccggggggt cttacaagct caccctgacg gagatctagt aatgaactag tccgtaactt
     1321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacag ataaatgaag
     1381 ggcatggact g
//
