LOCUS       sequence-27801-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mPA-GFP-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..452
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 tggacgagct gtacaaggga tccttggcta ctacacttga acgtattgag aagaactttg
       61 tcattactga cccaagattg ccagataatc ccattatatt cgcgtccgat agtttcttgc
      121 agttgacaga atatagccgt gaagaaattt tgggaagaaa ctgcaggttt ctacaaggtc
      181 ctgaaactga tcgcgcgaca gtgagaaaaa ttagagatgc catagataac caaacagagg
      241 tcactgttca gctgattaat tatacaaaga gtggtaaaaa gttctggaac ctctttcact
      301 tgcagcctat gcgagatcag aagggagatg tccagtactt tattggggtt cagttggatg
      361 gaactgagca tgtccgagat gctgccgaga gagagggagt catgctgatt aagaaaactg
      421 cagaaaatat tgatgaggcg gcaaaagaac ttatcaagtg tgtggtggtg ggagacggag
      481 ctgtaggtaa aacttgccta ctgatcagtt acacaaccaa tgcatttcct ggagaatata
      541 tccctactgt ctttgacaat tattctgcca atgttatggt agatggaaaa ccggtgaatc
      601 tgggcttatg ggatacagct ggactagaag attatgacag attacgcccc ctatcctatc
      661 cgcaaacaga tgtgttctta atttgctttt cccttgnnna gtcctgcatc attcc
//
