LOCUS       sequence-278014-        1769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2184
FEATURES             Location/Qualifiers
     source          1..1769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatggaga cgggttgaca ttcagggacg tggccatcga attctctcaa
       61 gatgagtgga aatgcctgaa ctctacacag aggactttat acagggatgt gatgttggag
      121 aactacagga acctggtctc cctggatctg tctcgtaact gtgtaatcaa ggaactagca
      181 ccacaacagg aaggtaaccc aggagaagta ttccacacag tgacattgga acaacatgaa
      241 aaacatgaca ttgaagagtt ttgcttcagg gaaatcaaga aaaaaataca cgactttgac
      301 tgtcagtgga gagatgatga aagaaattgc aacaaagtga ctacggcccc aaaagaaaat
      361 cttacttgta ggagagacca acgcgataga agaggtatag gaaacaagtc tattaaacat
      421 cagcttggat taagctttct accacatccc catgaactgc agcagtttca agctgaaggg
      481 aaaatttatg aatgtaacca tgttgagaag tctgtcaacc atggttcctc agtttcacca
      541 ccccaaataa tttcttctac catcaaaacc catgtttcta ataaatatgg gactgatttc
      601 atctgttctt cattactcac acaagaacag aaatcatgca ttagggaaaa accttacaga
      661 tatattgagt gcgacaaagc cttgaatcat ggctcacaca tgactgtacg tcaggtaagt
      721 cattctggag agaaaggata taaatgtgat ctgtgtggca aggtctttag tcaaaaatca
      781 aaccttgcgc gtcattggag agttcatact ggagagaaac catacaaatg taatgaatgt
      841 gacagaagtt tcagtcgcaa ctcatgcctt gcactacatc ggagagttca cactggagag
      901 aaaccttaca aatgttatga gtgtgacaag gtcttcagtc gaaattcatg ccttgcacta
      961 catcagaaaa ctcatattgg agagaaacct tacacatgta aagagtgtgg caaagccttt
     1021 agtgtgaggt caacacttac caaccatcag gtaattcata gtggcaagaa accttacaaa
     1081 tgcaatgaat gtggcaaggt gttcagtcag acttcaagcc ttgcaactca tcagagaatt
     1141 cacactgggg agaaaccata caagtgtaat gaatgtggta aagtcttcag tcagacttca
     1201 agccttgcaa ggcattggag aattcatact ggagagaaac cttacaaatg caatgaatgt
     1261 ggtaaggttt tcagttacaa ttcacacctt gcgagtcatc ggagagttca tactggagag
     1321 aaaccttaca agtgtaatga gtgtgggaaa gcctttagtg tgcattcgaa cttaactacc
     1381 catcaggtca tccatactgg agagaagcct tacaaatgta atcaatgtgg caaaggcttc
     1441 agtgtgcatt caagcctaac tacccatcag gtcatccata ctggagaaaa accttacaaa
     1501 tgtaatgagt gtggcaaatc ctttagtgtg cgcccaaacc tcactagaca tcagataatc
     1561 catactggaa agaaacctta caaatgtagt gattgtggga agtcctttag tgtgcgccca
     1621 aacctcttca gacatcaaat tatccatact aaggagaaac cttataaaag aaattagtaa
     1681 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1741 gacgaagatc ccaggacctc atcgacaat
//
