LOCUS       sequence-27802-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             629..661
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    675..691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(759..782)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttgatgaggc ggcaaagaac ttatcaagtg tgtggtggtg ggagacggag ctgtaggtaa
       61 aacttgccta ctgatcagtt acacaaccaa tgcatttcct ggagaatata tccctactgt
      121 ctttgacaat tattctgcca atgttatggt agatggaaaa ccggtgaatc tgggcttatg
      181 ggatacagct ggactagaag attatgacag attacgcccc ctatcctatc cgcaaacaga
      241 tgtgttctta atttgctttt cccttgtgag tcctgcatca ttccaccacg tccgtgcaaa
      301 gtggtatcct gaggtgcggc accactgtcc caacactccc atcatcctag tgggaactaa
      361 acttgatctt agggatgata aagacacgat cgagaaactg aaggagaaga agctgactcc
      421 catcacctat ccgcagggtc tagccatggc taaggagatt ggtgctgtaa aatacctgga
      481 gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt gacgaagcga tccgagcagt
      541 cctctgcccg cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt aaaagcttgc
      601 ggccgcacag ctgtatacac gtgcaagcca gccagaactc gccccggaag accccgagga
      661 tctcgagtgt ggaattgtga gcggataaca atttgtcgag ggcccctgca ggtcaattct
      721 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
      781 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
      841 ggcgccaacc ggctccgttc tttggt
//
