LOCUS       sequence-278059-        1289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2215
FEATURES             Location/Qualifiers
     source          1..1289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatctcgg aatccctgac ctgctggacg cgtggctgga gcccccagag
       61 gatatcttct cgacaggatc cgtcctggag ctgggactcc actgcccccc tccagaggtt
      121 ccggtaacta ggctacagga acagggactg caaggctgga agtccggtgg ggaccgtggc
      181 tgtggccttc aagagagtga gcctgaagat ttcttgaagc ttttcattga tcccaatgag
      241 gtgtactgct cagaagcatc tcctggcagt gacagtggca tctctgagga cccctgccat
      301 ccagacagtc cccctgcccc cagggcaacc agttctccta tgctctatga ggttgtctat
      361 gaggcagggg ccctggagag gatgcagggg gaaactgggc caaatgtagg ccttatctcc
      421 atccagctag atcagtggag cccagcattt atggtgcctg attcctgcat ggtcagtgag
      481 ctgccctttg atgctcatgc ccacatcctg cccagagcag gcaccgtagc cccagtgccc
      541 tgtacaaccc tgctgccctg tcaaaccctg ttcctgaccg atgaggagaa gcgtctgctg
      601 gggcaggaag gggtttccct gccctctcac ctgcccctca ccaaggcaga ggagagggtc
      661 ctcaagaagg tcaggaggaa aatccgtaac aagcagtcag ctcaggacag tcggcggcgg
      721 aagaaggagt acattgatgg gctggagagc agggtggcag cctgttctgc acagaaccaa
      781 gaattacaga aaaaagtcca ggagctggag aggcacaaca tctccttggt agctcagctc
      841 cgccagctgc agacgctaat tgctcaaact tccaacaaag ctgcccagac cagcacttgt
      901 gttttgattc ttcttttttc cctggctctc atcatcctgc ccagcttcag tccattccag
      961 agtcgaccag aagctgggtc tgaggattac cagcctcacg gagtgacttc cagaaatatc
     1021 ctgacccaca aggacgtaac agaaaatctg gagacccaag tggtagagtc cagactgagg
     1081 gagccacctg gagccaagga tgcaaatggc tcaacaagga cactgcttga gaagatggga
     1141 gggaagccaa gacccagtgg gcgcatccgg tccgtgctgc atgcagatga gatgtagtaa
     1201 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgaggcct ggtagtgtag tgtgcccgg
//
