LOCUS       sequence-278062-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2219
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcccgg gcagcagccg ccgcctcaac cggcccccca gggccaaggg
       61 cagccgcctt cgcagccccc gcaggggcag ggcccgccgt ccggacccgg gcaaccggca
      121 cccgcggcga cccaggcggc gccgcaggca ccccccgccg ggcatcagat cgtgcacgtc
      181 cgcggggact cggagaccga cctggaggcg ctcttcaacg ccgtcatgaa ccccaagacg
      241 gccaacgtgc cccagaccgt gcccatgagg ctccggaagc tgcccgactc cttcttcaag
      301 ccgccggagc ccaaatccca ctcccgacag gccagtactg atgcaggcac tgcaggagcc
      361 ctgactccac agcatgttcg agctcattcc tctccagctt ctctgcagtt gggagctgtt
      421 tctcctggga cactgacccc cactggagta gtctctggcc cagcagctac acccacagct
      481 cagcatcttc gacagtcttc ttttgagata cctgatgatg tacctctgcc agcaggttgg
      541 gagatggcaa agacatcttc tggtcagaga tacttcttaa atcacatcga tcagacaaca
      601 acatggcagg accccaggaa ggccatgctg tcccagatga acgtcacagc ccccaccagt
      661 ccaccagtgc agcagaatat gatgaactcg gcttcaggtc ctcttcctga tggatgggaa
      721 caagccatga ctcaggatgg agaaatttac tatataaacc ataagaacaa gaccacctct
      781 tggctagacc caaggcttga ccctcgtttt gccatgaacc agagaatcag tcagagtgct
      841 ccagtgaaac agccaccacc cctggctccc cagagcccac agggaggcgt catgggtggc
      901 agcaactcca accagcagca acagatgcga ctgcagcaac tgcagatgga gaaggagagg
      961 ctgcggctga aacagcaaga actgcttcgg caggcaatgc ggaatatcaa tcccagcaca
     1021 gcaaattctc caaaatgtca ggagttagcc ctgcgtagcc agttaccaac actggagcag
     1081 gatggtggga ctcaaaatcc agtgtcttct cccgggatgt ctcaggaatt gagaacaatg
     1141 acgaccaata gctcagatcc tttccttaac agtggcacct atcactctcg agatgagagt
     1201 acagacagtg gactaagcat gagcagctac agtgtccctc gaaccccaga tgacttcctg
     1261 aacagtgtgg atgagatgga tacaggtgat actatcaacc aaagcaccct gccctcacag
     1321 cagaaccgtt tcccagacta ccttgaagcc attcctggga caaatgtgga ccttggaaca
     1381 ctggaaggag atggaatgaa catagaagga gaggagctga tgccaagtct gcaggaagct
     1441 ttgagttctg acatccttaa tgacatggag tctgttttgg ctgccaccaa gctagataaa
     1501 gaaagctttc ttacatggtt atagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gatcttattg ttggatgagt gtctta
//
