LOCUS       sequence-27808-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Rac1 fused with light-insensitive LOV2 (C450A 
            mutation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-PA-Rac1-C450A
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             628..660
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             667..690
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 tgatgaggcg gcaaagaact tatcaagtgt gtggtggtgg gagacggagc tgtaggtaaa
       61 acttgcctac tgatcagtta cacaaccaat gcatttcctg gagaatatat ccctactgtc
      121 tttgacaatt attctgccaa tgttatggta gatggaaaac cggtgaatct gggcttatgg
      181 gatacagctg gactagaaga ttatgacaga ttacgccccc tatcctatcc gcaaacagat
      241 gtgttcttaa tttgcttttc ccttgtgagt cctgcatcat tccaccacgt ccgtgcaaag
      301 tggtatcctg aggtgcggca ccactgtccc aacactccca tcatcctagt gggaactaaa
      361 cttgatctta gggatgataa agacacgatc gagaaactga aggagaagaa gctgactccc
      421 atcacctatc cgcagggtct agccatggct aaggagattg gtgctgtaaa atacctggag
      481 tgctcggcgc tcacacagcg aggcctcaag acagtgtttg acgaagcgat ccgagcagtc
      541 ctctgcccgc ctcccgtgaa gaagaggaag agaaaatgcc tgctgttgta aaagcttgcg
      601 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
      661 ctcgagcacc accatcacca tcaccatcac taagtganta acctcaggtg caggctgcct
      721 atcagaagtg gtggctggtg tggcaa
//
