LOCUS       sequence-278080-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2233
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcccg ggcgggtttc cagtctgtgg ctccaagcgg cggcgccgga
       61 gcctcaggag gggcgggcgc ggctgctgcc ttgggcccgg gcggaactcc ggggcctcct
      121 gtgcgaatgg gcccggctcc gggtcaaggg ctgtaccgct ccccgatgcc cggagcggcc
      181 tatccgagac caggtatgtt gccaggcagc cgaatgacac ctcagggacc ttccatggga
      241 ccccctggct atggggggaa cccttcagtc cgacctggcc tggcccagtc agggatggat
      301 cagtcccgca agagacctgc ccctcagcag atccagcagg tccagcagca ggcggtccaa
      361 aatcgaaacc acaatgcaaa gaaaaagaag atggctgaca aaattctacc tcaaaggatt
      421 cgtgaactgg taccagaatc ccaggcctat atggatctct tggcttttga aaggaaactg
      481 gaccagacta tcatgaggaa acggctagat atccaagagg ccttgaaacg tcccatcaag
      541 caaaaacgga agctgcgaat tttcatttct aacactttca atccggctaa gtcagatgcc
      601 gaggatgggg aagggacggt ggcttcctgg gagcttcggg tagaaggacg gctcctggag
      661 gattcagcct tgtccaaata tgatgccact aaacaaaaga ggaagttctc ttcctttttt
      721 aagtccttgg tgattgaact ggacaaagac ctgtatgggc cagacaacca tctggtagaa
      781 tggcacagga ccgccactac ccaggagacc gatggctttc aggtgaagcg gccgggagac
      841 gtgaatgtac ggtgtactgt cctactgatg ctggattacc agcctcccca gtttaaatta
      901 gacccccgcc tagctcgact cctgggcatc catacccaga ctcgtccagt gatcatccaa
      961 gcactgtggc aatatattaa gacacataag ctccaggacc ctcacgagcg ggagtttgtc
     1021 atctgtgaca agtacctgca gcagatcttt gagtctcaac gtatgaagtt ttcagagatc
     1081 cctcagcggc tccatgcctt gcttatgcca ccagaaccta tcatcattaa tcatgtcatc
     1141 agtgttgacc cgaatgatca gaaaaagaca gcttgttatg acattgatgt tgaagtggat
     1201 gacaccttga agacccagat gaattctttt ctgctgtcca ctgccagcca acaggagatt
     1261 gctactctag acaacaagac aatgactgat gtggtgggta acccagagga ggagcgccga
     1321 gctgagttct acttccagcc ctgggctcag gaggctgtgt gccgatactt ctactccaag
     1381 gtgcagcaga gacgacaaga attagagcaa gccctgggaa tccggaatac atagtaatga
     1441 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1501 gaagtaccgg cttattaacc ttagtc
//
