LOCUS       sequence-27810-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 ('lit' state mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-PA-Rac1-I539E
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             635..667
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             674..697
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gagcnaagag agaggcggca aagaacttat caagtgtgtg gtggtgggag acggagctgt
       61 aggtaaaact tgcctactga tcagttacac aaccaatgca tttcctggag aatatatccc
      121 tactgtcttt gacaattatt ctgccaatgt tatggtagat ggaaaaccgg tgaatctggg
      181 cttatgggat acagctggac tagaagatta tgacagatta cgccccctat cctatccgca
      241 aacagatgtg ttcttaattt gcttttccct tgtgagtcct gcatcattcc accacgtccg
      301 tgcaaagtgg tatcctgagg tgcggcacca ctgtcccaac actcccatca tcctagtggg
      361 aactaaactt gatcttaggg atgataaaga cacgatcgag aaactgaagg agaagaagct
      421 gactcccatc acctatccgc agggtctagc catggctaag gagattggtg ctgtaaaata
      481 cctggagtgc tcggcgctca cacagcgagg cctcaagaca gtgtttgacg aagcgatccg
      541 agcagtcctc tgcccgcctc ccgtgaagaa gaggaataga aaatgcctgc tgttgtaaaa
      601 gcttgcggcc gcacagctgt atacacgtgc aagccagcca gaactcgccc cggaagaccc
      661 cgaggatctc gagcaccacc atcaccatca ccatcactaa gtgattaacc tcaggtgcag
      721 gctgcctatc aaaagtnctg gctggggcag cncagccccg ccaaaaanac aaaaanaaat
      781 ntttncccnc ncaaatatgg gaatnagacc ccttgactcg antcggtaaa agaattnttn
      841 tncttgttgt ggtt
//
