LOCUS       sequence-27811-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 ('lit' state mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-PA-Rac1-I539E
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..231
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     293..312
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     354..373
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        354..372
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    373..397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        413..522
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             543..560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagtcgttta gtgaaccgtc agatcactag atgctttatt gcggtagttt atcacaggtt
       61 aaattgctaa cgccaggtct cgaacttaac gtgcagaagt tggtcgtgag gcactgggca
      121 ggtaagtatc gggccctttg tgcgggggga gcggctcggg gctgtccgcg gggggacggc
      181 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
      241 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
      301 ctggttattg tgctgtctca tcattttggc aaagaattgg atcggaccga aattaatacg
      361 actcactata ggggaattgt gagcggataa caattccccg gagttaatcc gggaccttta
      421 attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc atttgtatat
      481 taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga tataccatgg
      541 cacaccatca ccaccatcac ggatccttgg ctactacact tgaacgtatt gagaagaact
      601 ttgtcattac tgacccaaga ttgccagata atcccattat attcgcgtcc gatagtttct
      661 tgcagttgac agaatatagc cgtgaagaaa ttttgggaag aaactgcagg tttctacaag
      721 gtcctgaaac tgatcgcgcg acagtgagaa aaattagaga tgccatagat aaccaaacag
      781 aggtcactgt tcagctgatt aattatacaa agagtggtaa aaagttctgg aacctctttc
      841 acttgcagcc tatgcgagat cagaa
//
