LOCUS       sequence-27813-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..227
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     289..308
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     350..369
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        350..368
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    369..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        409..518
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             539..556
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(705..723)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtcgtttagt gaaccgtcag atcactagat gctttattgc ggtagtttat cacagttaaa
       61 ttgctaacgc cagtctcgaa cttaacgtgc agaagttggt cgtgaggcac tgggcaggta
      121 agtatcgggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
      181 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
      241 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
      301 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
      361 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
      421 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
      481 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggcaca
      541 ccatcaccac catcacggat ctgtgagcaa gggcgaggag gataacatgg ccatcatcaa
      601 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      661 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      721 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      781 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      841 gggcttcaag tgggagcgcg tgatgaactt c
//
