LOCUS       sequence-278131-        1346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2260
FEATURES             Location/Qualifiers
     source          1..1346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             958..975
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             964..981
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
ORIGIN
        1 accgccacca tgagcagccc ggatgcggga tacgccagtg acgaccagag ccagacccag
       61 agcgcgctgc ccgcggtgat ggccgggctg ggcccctgcc cctgggccga gtcgctgagc
      121 cccatcgggg acatgaaggt gaagggcgag gcgccggcga acagcggagc accggccggg
      181 gccgcgggcc gagccaaggg cgagtcccgt atccggcggc cgatgaacgc tttcatggtg
      241 tgggctaagg acgagcgcaa gcggctggcg cagcagaatc cagacctgca caacgccgag
      301 ttgagcaaga tgctgggcaa gtcgtggaag gcgctgacgc tggcggagaa gcggcccttc
      361 gtggaggagg cagagcggct gcgcgtgcag cacatgcagg accaccccaa ctacaagtac
      421 cggccgcggc ggcgcaagca ggtgaagcgg ctgaagcggg tggagggcgg cttcctgcac
      481 ggcctggctg agccgcaggc ggccgcgctg ggccccgagg gcggccgcgt ggccatggac
      541 ggcctgggcc tccagttccc cgagcagggc ttccccgccg gcccgccgct gctgcctccg
      601 cacatgggcg gccactaccg cgactgccag agtctgggcg cgcctccgct cgacggctac
      661 ccgttgccca cgcccgacac gtccccgctg gacggcgtgg accccgaccc ggctttcttc
      721 gccgccccga tgcccgggga ctgcccggcg gccggcacct acagctacgc gcaggtctcg
      781 gactacgctg gccccccgga gcctcccgcc ggtcccatgc acccccgact cggcccagag
      841 cccgcgggtc cctcgattcc gggcctcctg gcgccaccca gcgcccttca cgtgtactac
      901 ggcgcgatgg gctcgcccgg ggcgggcggc gggcgcggct tccagatgca gccgcaacac
      961 cagcaccagc accagcacca gcaccacccc ccgggccccg gacagccgtc gccccctccg
     1021 gaggcactgc cctgccggga cggcacggac cccagtcagc ccgccgagct cctcggggag
     1081 gtggaccgca cggaatttga acagtatctg cacttcgtgt gcaagcctga gatgggcctc
     1141 ccctaccagg ggcatgactc cggtgtgaat ctccccgaca gccacggggc catttcctcg
     1201 gtggtgtccg acgccagctc cgcggtatat tactgcaact atcctgacgt gtagtaatga
     1261 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1321 gacaacctca caggatatag catatt
//
