LOCUS       sequence-27818-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 (dominant-negative mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCherry-PA-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             627..659
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             666..689
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ttgatgaggc ggcaaagaac ttatcaagtg tgtggtggtg ggagacggag ctgtaggtaa
       61 aaattgccta ctgatcagtt acacaaccaa tgcatttcct ggagaatata tccctactgt
      121 ctttgacaat tattctgcca atgttatggt agatggaaaa ccggtgaatc tgggcttatg
      181 ggatacagct ggacaagaag attatgacag attacgcccc ctatcctatc cgcaaacaga
      241 tgtgttctta atttgctttt cccttgtgag tcctgcatca tttgaaaatg tccgtgcaaa
      301 gtggtatcct gaggtgcggc accactgtcc caacactccc atcatcctag tgggaactaa
      361 acttgatctt agggatgata aagacacgat cgagaaactg aaggagaaga agctgactcc
      421 catcacctat ccgcagggtc tagcaatggc taaggagatt ggtgctgtaa aatacctgga
      481 gtgctcggcg ctcacacagc gaggcctcaa gacagtgttt gacgaagcga tccgagcagt
      541 cctctgcccg cctcccgtga agaagaggaa gagaaaatgc ctgctgttgt aagcttgcgg
      601 ccgcacagct gtatacacgt gcaagccagc cagaactcgc cccggaagac cccgaggatc
      661 tcgagcacca ccatcaccat caccatcact aagtgattaa cctcaggtgc aggctgccta
      721 t
//
