LOCUS       sequence-27819-e         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 (dominant-negative mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCherry-PA-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..226
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     288..307
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     349..368
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        349..367
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    368..392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        408..517
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             538..555
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtcgtttagt gaccgtcaga tcactagatg ctttattgcg gtagtttatc acagttaaat
       61 tgctaacgcc agtctcgaac ttaacgtgca gaagttggtc gtgaggcact gggcaggtaa
      121 gtatcgggcc ctttgtgcgg ggggagcggc tcggggctgt ccgcgggggg acggctgcct
      181 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
      241 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
      301 tattgtgctg tctcatcatt ttggcaaaga attggatcgg accgaaatta atacgactca
      361 ctatagggga attgtgagcg gataacaatt ccccggagtt aatccgggac ctttaattca
      421 acccaacaca atatattata gttaaataag aattattatc aaatcatttg tatattaatt
      481 aaaatactat actnnaaatt acattttatt tacaatcaaa ggagatatac catggcacac
      541 catcaccacc atcacggatc tgtgagcaag ggcgaggagg ataacatggc catcatcaag
      601 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      661 gagggcga
//
