LOCUS       sequence-278191-        3494 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2302
FEATURES             Location/Qualifiers
     source          1..3494
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcgttgcct ggctccacgc cctgctgggt cctacctgtc agagccccaa
       61 ggcagctcac agtgtgccac catggagttg gggcccctag aaggtggcta cctggagctt
      121 cttaacagcg atgctgaccc cctgtgcctc taccacttct atgaccagat ggacctggct
      181 ggagaagaag agattgagct ctactcagaa cccgacacag acaccatcaa ctgcgaccag
      241 ttcagcaggc tgttgtgtga catggaaggt gatgaagaga ccagggaggc ttatgccaat
      301 atcgcggaac tggaccagta tgtcttccag gactcccagc tggagggcct gagcaaggac
      361 attttcaagc acataggacc agatgaagtg atcggtgaga gtatggagat gccagcagaa
      421 gttgggcaga aaagtcagaa aagacccttc ccagaggagc ttccggcaga cctgaagcac
      481 tggaagccag ctgagccccc cactgtggtg actggcagtc tcctagtggg accagtgagc
      541 gactgctcca ccctgccctg cctgccactg cctgcgctgt tcaaccagga gccagcctcc
      601 ggccagatgc gcctggagaa aaccgaccag attcccatgc ctttctccag ttcctcgttg
      661 agctgcctga atctccctga gggacccatc cagtttgtcc ccaccatctc cactctgccc
      721 catgggctct ggcaaatctc tgaggctgga acaggggtct ccagtatatt catctaccat
      781 ggtgaggtgc cccaggccag ccaagtaccc cctcccagtg gattcactgt ccacggcctc
      841 ccaacatctc cagaccggcc aggctccacc agccccttcg ctccatcagc cactgacctg
      901 cccagcatgc ctgaacctgc cctgacctcc cgagcaaaca tgacagagca caagacgtcc
      961 cccacccaat gcccggcagc tggagaggtc tccaacaagc ttccaaaatg gcctgagccg
     1021 gtggagcagt tctaccgctc actgcaggac acgtatggtg ccgagcccgc aggcccggat
     1081 ggcatcctag tggaggtgga tctggtgcag gccaggctgg agaggagcag cagcaagagc
     1141 ctggagcggg aactggccac cccggactgg gcagaacggc agctggccca aggaggcctg
     1201 gctgaggtgc tgttggctgc caaggagcac cggcggccgc gtgagacacg agtgattgct
     1261 gtgctgggca aagctggtca gggcaagagc tattgggctg gggcagtgag ccgggcctgg
     1321 gcttgtggcc ggcttcccca gtacgacttt gtcttctctg tcccctgcca ttgcttgaac
     1381 cgtccggggg atgcctatgg cctgcaggat ctgctcttct ccctgggccc acagccactc
     1441 gtggcggccg atgaggtttt cagccacatc ttgaagagac ctgaccgcgt tctgctcatc
     1501 ctagacggct tcgaggagct ggaagcgcaa gatggcttcc tgcacagcac gtgcggaccg
     1561 gcaccggcgg agccctgctc cctccggggg ctgctggccg gccttttcca gaagaagctg
     1621 ctccgaggtt gcaccctcct cctcacagcc cggccccggg gccgcctggt ccagagcctg
     1681 agcaaggccg acgccctatt tgagctgtcc ggcttctcca tggagcaggc ccaggcatac
     1741 gtgatgcgct actttgagag ctcagggatg acagagcacc aagacagagc cctgacgctc
     1801 ctccgggacc ggccacttct tctcagtcac agccacagcc ctactttgtg ccgggcagtg
     1861 tgccagctct cagaggccct gctggagctt ggggaggacg ccaagctgcc ctccacgctc
     1921 acgggactct atgtcggcct gctgggccgt gcagccctcg acagcccccc cggggccctg
     1981 gcagagctgg ccaagctggc ctgggagctg ggccgcagac atcaaagtac cctacaggag
     2041 gaccagttcc catccgcaga cgtgaggacc tgggcgatgg ccaaaggctt agtccaacac
     2101 ccaccgcggg ccgcagagtc cgagctggcc ttccccagct tcctcctgca atgcttcctg
     2161 ggggccctgt ggctggctct gagtggcgaa atcaaggaca aggagctccc gcagtaccta
     2221 gcattgaccc caaggaagaa gaggccctat gacaactggc tggagggcgt gccacgcttt
     2281 ctggctgggc tgatcttcca gcctcccgcc cgctgcctgg gagccctact cgggccatcg
     2341 gcggctgcct cggtggacag gaagcagaag gtgcttgcga ggtacctgaa gcggctgcag
     2401 ccggggacac tgcgggcgcg gcagctgctg gagctgctgc actgcgccca cgaggccgag
     2461 gaggctggaa tttggcagca cgtggtacag gagctccccg gccgcctctc ttttctgggc
     2521 acccgcctca cgcctcctga tgcacatgta ctgggcaagg ccttggaggc ggcgggccaa
     2581 gacttctccc tggacctccg cagcactggc atttgcccct ctggattggg gagcctcgtg
     2641 ggactcagct gtgtcacccg tttcagggct gccttgagcg acacggtggc gctgtgggag
     2701 tccctgcagc agcatgggga gaccaagcta cttcaggcag cagaggagaa gttcaccatc
     2761 gagcctttca aagccaagtc cctgaaggat gtggaagacc tgggaaagct tgtgcagact
     2821 cagaggacga gaagttcctc ggaagacaca gctggggagc tccctgctgt tcgggaccta
     2881 aagaaactgg agtttgcgct gggccctgtc tcaggccccc aggctttccc caaactggtg
     2941 cggatcctca cggccttttc ctccctgcag catctggacc tggatgcgct gagtgagaac
     3001 aagatcgggg acgagggtgt ctcgcagctc tcagccacct tcccccagct gaagtccttg
     3061 gaaaccctca atctgtccca gaacaacatc actgacctgg gtgcctacaa actcgccgag
     3121 gccctgcctt cgctcgctgc atccctgctc aggctaagct tgtacaataa ctgcatctgc
     3181 gacgtgggag ccgagagctt ggctcgtgtg cttccggaca tggtgtccct ccgggtgatg
     3241 gacgtccagt acaacaagtt cacggctgcc ggggcccagc agctcgctgc cagccttcgg
     3301 aggtgtcctc atgtggagac gctggcgatg tggacgccca ccatcccatt cagtgtccag
     3361 gaacacctgc aacaacagga ttcacggatc agcctgagat agtaatgaac tagtccgtaa
     3421 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aattctcata
     3481 ctatccgctg tcga
//
