LOCUS       sequence-278197-        3497 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2304
FEATURES             Location/Qualifiers
     source          1..3497
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgcgttgcct ggctccacgc cctgctgggt cctacctgtc agagccccaa
       61 ggcagctcac agtgtgccac catggagttg gggcccctag aaggtggcta cctggagctt
      121 cttaacagcg atgctgaccc cctgtgcctc taccacttct atgaccagat ggacctggct
      181 ggagaagaag agattgagct ctactcagaa cccgacacag acaccatcaa ctgcgaccag
      241 ttcagcaggc tgttgtgtga catggaaggt gatgaagaga ccagggaggc ttatgccaat
      301 atcgcggaac tggaccagta tgtcttccag gactcccagc tggagggcct gagcaaggac
      361 attttcatag agcacatagg accagatgaa gtgatcggtg agagtatgga gatgccagca
      421 gaagttgggc agaaaagtca gaaaagaccc ttcccagagg agcttccggc agacctgaag
      481 cactggaagc cagctgagcc ccccactgtg gtgactggca gtctcctagt gggaccagtg
      541 agcgactgct ccaccctgcc ctgcctgcca ctgcctgcgc tgttcaacca ggagccagcc
      601 tccggccaga tgcgcctgga gaaaaccgac cagattccca tgcctttctc cagttcctcg
      661 ttgagctgcc tgaatctccc tgagggaccc atccagtttg tccccaccat ctccactctg
      721 ccccatgggc tctggcaaat ctctgaggct ggaacagggg tctccagtat attcatctac
      781 catggtgagg tgccccaggc cagccaagta ccccctccca gtggattcac tgtccacggc
      841 ctcccaacat ctccagaccg gccaggctcc accagcccct tcgctccatc agccactgac
      901 ctgcccagca tgcctgaacc tgccctgacc tcccgagcaa acatgacaga gcacaagacg
      961 tcccccaccc aatgcccggc agctggagag gtctccaaca agcttccaaa atggcctgag
     1021 ccggtggagc agttctaccg ctcactgcag gacacgtatg gtgccgagcc cgcaggcccg
     1081 gatggcatcc tagtggaggt ggatctggtg caggccaggc tggagaggag cagcagcaag
     1141 agcctggagc gggaactggc caccccggac tgggcagaac ggcagctggc ccaaggaggc
     1201 ctggctgagg tgctgttggc tgccaaggag caccggcggc cgcgtgagac acgagtgatt
     1261 gctgtgctgg gcaaagctgg tcagggcaag agctattggg ctggggcagt gagccgggcc
     1321 tgggcttgtg gccggcttcc ccagtacgac tttgtcttct ctgtcccctg ccattgcttg
     1381 aaccgtccgg gggatgccta tggcctgcag gatctgctct tctccctggg cccacagcca
     1441 ctcgtggcgg ccgatgaggt tttcagccac atcttgaaga gacctgaccg cgttctgctc
     1501 atcctagacg gcttcgagga gctggaagcg caagatggct tcctgcacag cacgtgcgga
     1561 ccggcaccgg cggagccctg ctccctccgg gggctgctgg ccggcctttt ccagaagaag
     1621 ctgctccgag gttgcaccct cctcctcaca gcccggcccc ggggccgcct ggtccagagc
     1681 ctgagcaagg ccgacgccct atttgagctg tccggcttct ccatggagca ggcccaggca
     1741 tacgtgatgc gctactttga gagctcaggg atgacagagc accaagacag agccctgacg
     1801 ctcctccggg accggccact tcttctcagt cacagccaca gccctacttt gtgccgggca
     1861 gtgtgccagc tctcagaggc cctgctggag cttggggagg acgccaagct gccctccacg
     1921 ctcacgggac tctatgtcgg cctgctgggc cgtgcagccc tcgacagccc ccccggggcc
     1981 ctggcagagc tggccaagct ggcctgggag ctgggccgca gacatcaaag taccctacag
     2041 gaggaccagt tcccatccgc agacgtgagg acctgggcga tggccaaagg cttagtccaa
     2101 cacccaccgc gggccgcaga gtccgagctg gccttcccca gcttcctcct gcaatgcttc
     2161 ctgggggccc tgtggctggc tctgagtggc gaaatcaagg acaaggagct cccgcagtac
     2221 ctagcattga ccccaaggaa gaagaggccc tatgacaact ggctggaggg cgtgccacgc
     2281 tttctggctg ggctgatctt ccagcctccc gcccgctgcc tgggagccct actcgggcca
     2341 tcggcggctg cctcggtgga caggaagcag aaggtgcttg cgaggtacct gaagcggctg
     2401 cagccgggga cactgcgggc gcggcagctg ctggagctgc tgcactgcgc ccacgaggcc
     2461 gaggaggctg gaatttggca gcacgtggta caggagctcc ccggccgcct ctcttttctg
     2521 ggcacccgcc tcacgcctcc tgatgcacat gtactgggca aggccttgga ggcggcgggc
     2581 caagacttct ccctggacct ccgcagcact ggcatttgcc cctctggatt ggggagcctc
     2641 gtgggactca gctgtgtcac ccgtttcagg gctgccttga gcgacacggt ggcgctgtgg
     2701 gagtccctgc agcagcatgg ggagaccaag ctacttcagg cagcagagga gaagttcacc
     2761 atcgagcctt tcaaagccaa gtccctgaag gatgtggaag acctgggaaa gcttgtgcag
     2821 actcagagga cgagaagttc ctcggaagac acagctgggg agctccctgc tgttcgggac
     2881 ctaaagaaac tggagtttgc gctgggccct gtctcaggcc cccaggcttt ccccaaactg
     2941 gtgcggatcc tcacggcctt ttcctccctg cagcatctgg acctggatgc gctgagtgag
     3001 aacaagatcg gggacgaggg tgtctcgcag ctctcagcca ccttccccca gctgaagtcc
     3061 ttggaaaccc tcaatctgtc ccagaacaac atcactgacc tgggtgccta caaactcgcc
     3121 gaggccctgc cttcgctcgc tgcatccctg ctcaggctaa gcttgtacaa taactgcatc
     3181 tgcgacgtgg gagccgagag cttggctcgt gtgcttccgg acatggtgtc cctccgggtg
     3241 atggacgtcc agtacaacaa gttcacggct gccggggccc agcagctcgc tgccagcctt
     3301 cggaggtgtc ctcatgtgga gacgctggcg atgtggacgc ccaccatccc attcagtgtc
     3361 caggaacacc tgcaacaaca ggattcacgg atcagcctga gatagtaatg aactagtccg
     3421 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgattcgctt
     3481 gtacgggtca cgtttga
//
