LOCUS       sequence-27820-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1 (dominant-negative mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCherry-PA-Rac1-T17N
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..676
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 ccccgaggan ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gggatccttg gctactacac ttgaacgtat tgagaagaac tttgtcatta ctgacccaag
      301 attgccagat aatcccatta tattcgcgtc cgatagtttc ttgcagttga cagaatatag
      361 ccgtgaagaa attttgggaa gaaactgcag gtttctacaa ggtcctgaaa ctgatcgcgc
      421 gacagtgaga aaaattagag atgccataga taaccaaaca gaggtcactg ttcagctgat
      481 taattataca aagagtggta aaaagttctg gaacctcttt cacttgcagc ctatgcgaga
      541 tcagaaggga gatgtccagt actttattgg ggttcagttg gatggaactg agcatgtccg
      601 agatgctgcc gagagagagg gagtcatgct gattaagaaa actgcagaaa atattgatga
      661 ggcggcaaaa gaacttatca agtgtgtggt ggtgggagac ggagctgtag gtaaaaattg
      721 cctactgatc agttacacaa ccnatgcatt tcctgganaa tatatcccta c
//
