LOCUS       sequence-27823-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pTriEx-mCerulean-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..106
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             128..145
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(194..215)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(455..474)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctttaattca acccaacaca atatattata gttaaataag aattattatc aaatcatttg
       61 tatattaatt aaaatactat actgtaaatt acattttatt tacaatcaaa ggagatatac
      121 catggcacac catcaccacc atcacggttc tgtgagcaag ggcgaggagc tgttcaccgg
      181 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      241 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tcagcaccac
      301 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg
      361 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      421 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      481 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      541 caaggaggac ggcaacatcc tggggnacaa gctgg
//
