LOCUS       sequence-278230-        1556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2316
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggag gatgaaagcc ggatacttgg accagcaagt gccctacacc
       61 ttcagcagca aatcgcccgg aaatgggagc ttgcgcgaag cgctgatcgg cccgctgggg
      121 aagctcatgg acccgggctc cctgccgccc ctcgactctg aagatctctt ccaggatcta
      181 agtcacttcc aggagacgtg gctcgctgaa gctcaggtac cagacagtga tgagcagttt
      241 gttcctgatt tccattcaga aaacctagct ttccacagcc ccaccaccag gatcaagaag
      301 gagccccaga gtccccgcac agacccggcc ctgtcctgca gcaggaagcc gccactcccc
      361 taccaccatg gcgagcagtg cctttactcc agtgcctatg acccccccag acaaatcgcc
      421 atcaagtccc ctgcccctgg tgcccttgga cagtcgcccc tacagccctt tccccgggca
      481 gagcaacgga atttcctgag atcctctggc acctcccagc cccaccctgg ccatgggtac
      541 ctcggggaac atagctccgt cttccagcag cccctggaca tttgccactc cttcacatct
      601 cagggagggg gccgggaacc cctcccagcc ccctaccaac accagctgtc ggagccctgc
      661 ccaccctatc cccagcagag ctttaagcaa gaataccatg atcccctgta tgaacaggcg
      721 ggccagccag ccgtggacca gggtggggtc aatgggcaca ggtacccagg ggcgggggtg
      781 gtgatcaaac aggaacagac ggacttcgcc tacgactcag atgtcaccgg gtgcgcatca
      841 atgtacctcc acacagaggg cttctctggg ccctctccag gtgacggggc catgggctat
      901 ggctatgaga aacctctgcg accattccca gatgatgtct gcgttgtccc tgagaaattt
      961 gaaggagaca tcaagcagga aggggtcggt gcatttcgag aggggccgcc ctaccagcgc
     1021 cggggtgccc tgcagctgtg gcaatttctg gtggccttgc tggatgaccc aacaaatgcc
     1081 catttcattg cctggacggg ccggggaatg gagttcaagc tcattgagcc tgaggaggtc
     1141 gccaggctct ggggcatcca gaagaaccgg ccagccatga attacgacaa gctgagccgc
     1201 tcgctccgat actattatga gaaaggcatc atgcagaagg tggctggtga gcgttacgtg
     1261 tacaagtttg tgtgtgagcc cgaggccctc ttctctttgg ccttcccgga caatcagcgt
     1321 ccagctctca aggctgagtt tgaccggcct gtcagtgagg aggacacagt ccctttgtcc
     1381 cacttggatg agagccccgc ctacctccca gagctggctg gccccgccca gccatttggc
     1441 cccaagggtg gctactctta ctagtaatga actagtccgt aacttgaaag tatttcgatt
     1501 tcttggcttt atatatcttg tggaaaggac gatggtccgg gactgagaag tcctgc
//
