LOCUS       sequence-278233-        1868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2319
FEATURES             Location/Qualifiers
     source          1..1868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacctcga ggaggcggaa gagtttaaag aacgctgtac tcagtgtgct
       61 gctgtctcat ggggtcttac tgatgaaggc aaatattatt gcacttcttg ccacaatgtt
      121 acagagagat atcaggaagt tacaaacact gatcttattc ctaataccca aataaaagcc
      181 ctcaaccggg ggcttaaaaa aaaaaacaat actgaaaaag gctgggattg gtatgtgtgt
      241 gaaggtttcc agtatattct ttatcaacaa gcagaagcct taaagaacct tggagtaggc
      301 ccagagttaa agaacgatgt tttacataat ttttggaagc gctaccttca gaagagcaag
      361 caggcatatt gtaagaaccc agtttatacc actggaagga aacctacggt attagaagat
      421 aatctaagtc attcagactg ggctagtgag cctgagctgc taagtgatgt cagctgtcct
      481 ccttttcttg aaagtggagc ggagtctcag tctgacatcc acactcgaaa acctttcccc
      541 gtcagcaaag catcacaatc agaaacgtct gtctgctctg gatctctgga tggagttgaa
      601 tactcacaac gaaaggagaa gggaatcgtg aagatgacca tgccacagac acttgccttc
      661 tgttatctgt ccttactttg gcagagagaa gcaataacac tttcagatct tttgaggttt
      721 gttgaagagg accatattcc ttacataaat gcttttcagc attttccaga acagatgaaa
      781 ttatatggac gtgacagagg aatctttggt atagagtctt ggcctgacta cgaggacatc
      841 tacaaaaaaa cagtagaagt tggaacattt ttagatttgc ctcgttttcc agacataact
      901 gaagactgct atcttcatcc caacatactg tgtatgaaat acttgatgga agtcaacctc
      961 cctgatgaaa tgcatagctt aacttgccac gtggtaaaaa tgactggaat gggagaagtg
     1021 gattttctga catttgatcc tatagctaaa atggcaaaaa ctgttaagta cgatgtacaa
     1081 gctgtagcta tcattgtggt ggtattgaaa ctgctctttc tattggatga cagtttcgag
     1141 tggtctttgt ctaatcttgc tgaaaagcat aatgaaaaga acaaaaaaga taagccatgg
     1201 tttgatttca gaaagtggta ccaaattatg aagaaagctt ttgatgagaa aaaacaaaaa
     1261 tgggaagaag caagggccaa gtacctgtgg aaaagtgaaa agccactcta ctactcattt
     1321 gtcgacaaac cagtagcata taaaaaaaga gaaatggtgg tgaatctaca gaaacaattt
     1381 agcacactgg tcgagtcaac agcaactgct ggaaaaaaaa gcccttcaag ttttcagttc
     1441 aactggactg aagaggacac tgatagaacg tgtttccatg gacacagcct tcagggagtc
     1501 ctgaaagaga aaggccaatc actgctgact aagaattcat tatattggct tagtacacag
     1561 aaattctgca gatgctattg tacacatgtg acaacctatg aagaatcaaa ttattctctg
     1621 agttatcagt ttatactaaa tctcttctcc ttcctgctca gaataaagac ttcccttctc
     1681 catgaagaag tgagcttagt tgagaagaaa ctttttgaga aaaaatacag tgtaaaaaga
     1741 aagaaatcaa gatccaagaa agtgagacga cattagtaat gaactagtcc gtaacttgaa
     1801 agtatttcga tttcttggct ttatatatct tgtggaaagg acgagtcggt cgctcttcag
     1861 ttgttctt
//
