LOCUS       sequence-27824-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCerulean-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             637..669
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     protein_bind    683..699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gagcnaattt gatgaggcgg caaagaactt atcaagtgtg tggtggtggg agacggagct
       61 gtaggtaaaa cttgcctact gatcagttac acaaccaatg catttcctgg agaatatatc
      121 cctactgtct ttgacaatta ttctgccaat gttatggtag atggaaaacc ggtgaatctg
      181 ggcttatggg atacagctgg actagaagat tatgacagat tacgccccct atcctatccg
      241 caaacagatg tgttcttaat ttgcttttcc cttgtgagtc ctgcatcatt ccaccacgtc
      301 cgtgcaaagt ggtatcctga ggtgcggcac cactgtccca acactcccat catcctagtg
      361 ggaactaaac ttgatcttag ggatgataaa gacacgatcg agaaactgaa ggagaagaag
      421 ctgactccca tcacctatcc gcagggtcta gccatggcta aggagattgg tgctgtaaaa
      481 tacctggagt gctcggcgct cacacagcga ggcctcaaga cagtgtttga cgaagcgatc
      541 cgagcagtcc tctgcccgcc tcccgtgaag aagaggaaga gaaaatgcct gctgttgtaa
      601 aagcttgcgg ccgcacagct gtatacacgt gcaagccagc cagaactcgc cccggaagac
      661 cccgaggatc tcgagtgtgg aattgtgagc ggataacaat ttgtcgaggg cccctgcagn
      721 gctattctac gggtanggga gngcntttnc ccagganctg ngnacngcnt taacccccnn
      781 cggnattgnc ccacaanngg cntgnccccn nattcaaccc ccgggggcca cggcnct
//
