LOCUS       sequence-278242-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2333
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagagag actctttgag agtgcagaag gtagtcagtg tggagaaact
       61 ttcacccagg ttccggaaga catgctgaac aagaaaactc ttcctggagt aaaatcatgt
      121 gaaagtggta catgtggaga aatcttcatg ggatattcat cctttaatag gaacatcaga
      181 actgacactg gacaccaacc acataagtgt cagaaatttt tagagaagcc atataaacat
      241 aaacaacgta ggaaagcctt gagccatagc cactgctttc gaacacatga aaggcctcac
      301 actagagaga aaccttttga ttgtaaggaa tgtgaaaaat ctttcatttc tcctgcaagc
      361 attcgaagat atatggtaac gcacagtgga gatggacctt ataaatgtaa gttttgtggg
      421 aaagccttgg attgtctcag tttatacctt acccatgaac gaactcacac tggagagaaa
      481 cgatatgaat gtaaacaatg tggtaaagcc ttcagttggc acagttctgt tcgaatccat
      541 gaaagaactc acactgggga gaagccatat gaatgtaagg agtgtgggaa atcattcaat
      601 ttttccagtt cctttcgcag acatgaaagg acacacacag gagagaagcc gtacaaatgt
      661 aaggaatgtg ggaaagcctt caattgtccc agttcttttc acaggcatga aaggactcac
      721 acaggagaaa aaccctatga atgtaaacta tatgggaaag cattatctcg ccttataagc
      781 tttcgaagac acatgagaat gcacactgga gagaggcctc ataaatgtaa gatatgtggg
      841 aaagcctttt actctcccag ttcatttcaa aggcatgaaa gaagtcacac tggagagaaa
      901 ccctacaaat gcaagcaatg tgggaaagcc ttcacttgtt ccacttcgtt tcaatatcat
      961 gaaaggactc acactggaga gaaacccgat gggtgtaagc aatgtgggaa agccttcaga
     1021 tctgccaagt acattcgaat acatggaagg actcacactg gtgagaaacc ctatgaatgt
     1081 aagcaatgtg ggaaagcatt tcattgtgtc agctcctttc atagacatga aaggactcac
     1141 gctggagaaa aaccttatga atgtaagcat tgtgggaaag ccttcacttg ttccatatat
     1201 attagaatac atgaaagaat tcacactgga gagaaacctt accaatgtaa ggaatgtggg
     1261 aaagccttca ttcgttccag ttactgtcga aaacatgaaa gaactcacac tattaatata
     1321 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1381 ggaaaggacg aacctttcga atatgttcga tgaga
//
