LOCUS       sequence-278245-        2447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2334
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10..2352
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
ORIGIN
        1 accgccacca tggctactca agctgatttg atggagttgg acatggccat ggaaccagac
       61 agaaaagcgg ctgttagtca ctggcagcaa cagtcttacc tggactctgg aatccattct
      121 ggtgccacta ccacagctcc ttctctgagt ggtaaaggca atcctgagga agaggatgtg
      181 gatacctccc aagtcctgta tgagtgggaa cagggatttt ctcagtcctt cactcaagaa
      241 caagtagctg atattgatgg acagtatgca atgactcgag ctcagagggt acgagctgct
      301 atgttccctg agacattaga tgagggcatg cagatcccat ctacacagtt tgatgctgct
      361 catcccacta atgtccagcg tttggctgaa ccatcacaga tgctgaaaca tgcagttgta
      421 aacttgatta actatcaaga tgatgcagaa cttgccacac gtgcaatccc tgaactgaca
      481 aaactgctaa atgacgagga ccaggtggtg gttaataagg ctgcagttat ggtccatcag
      541 ctttctaaaa aggaagcttc cagacacgct atcatgcgtt ctcctcagat ggtgtctgct
      601 attgtacgta ccatgcagaa tacaaatgat gtagaaacag ctcgttgtac cgctgggacc
      661 ttgcataacc tttcccatca tcgtgagggc ttactggcca tctttaagtc tggaggcatt
      721 cctgccctgg tgaaaatgct tggttcacca gtggattctg tgttgtttta tgccattaca
      781 actctccaca accttttatt acatcaagaa ggagctaaaa tggcagtgcg tttagctggt
      841 gggctgcaga aaatggttgc cttgctcaac aaaacaaatg ttaaattctt ggctattacg
      901 acagactgcc ttcaaatttt agcttatggc aaccaagaaa gcaagctcat catactggct
      961 agtggtggac cccaagcttt agtaaatata atgaggacct atacttacga aaaactactg
     1021 tggaccacaa gcagagtgct gaaggtgcta tctgtctgct ctagtaataa gccggctatt
     1081 gtagaagctg gtggaatgca agctttagga cttcacctga cagatccaag tcaacgtctt
     1141 gttcagaact gtctttggac tctcaggaat ctttcagatg ctgcaactaa acaggaaggg
     1201 atggaaggtc tccttgggac tcttgttcag cttctgggtt cagatgatat aaatgtggtc
     1261 acctgtgcag ctggaattct ttctaacctc acttgcaata attataagaa caagatgatg
     1321 gtctgccaag tgggtggtat agaggctctt gtgcgtactg tccttcgggc tggtgacagg
     1381 gaagacatca ctgagcctgc catctgtgct cttcgtcatc tgaccagccg acaccaagaa
     1441 gcagagatgg cccagaatgc agttcgcctt cactatggac taccagttgt ggttaagctc
     1501 ttacacccac catcccactg gcctctgata aaggctactg ttggattgat tcgaaatctt
     1561 gccctttgtc ccgcaaatca tgcacctttg cgtgagcagg gtgccattcc acgactagtt
     1621 cagttgcttg ttcgtgcaca tcaggatacc cagcgccgta cgtccatggg tgggacacag
     1681 cagcaatttg tggagggggt ccgcatggaa gaaatagttg aaggttgtac cggagccctt
     1741 cacatcctag ctcgggatgt tcacaaccga attgttatca gaggactaaa taccattcca
     1801 ttgtttgtgc agctgcttta ttctcccatt gaaaacatcc aaagagtagc tgcaggggtc
     1861 ctctgtgaac ttgctcagga caaggaagct gcagaagcta ttgaagctga gggagccaca
     1921 gctcctctga cagagttact tcactctagg aatgaaggtg tggcgacata tgcagctgct
     1981 gttttgttcc gaatgtctga ggacaagcca caagattaca agaaacggct ttcagttgag
     2041 ctgaccagct ctctcttcag aacagagcca atggcttgga atgagactgc tgatcttgga
     2101 cttgatattg gtgcccaggg agaacccctt ggatatcgcc aggatgatcc tagctatcgt
     2161 tcttttcact ctggtggata tggccaggat gccttgggta tggaccccat gatggaacat
     2221 gagatgggtg gccaccaccc tggtgctgac tatccagttg atgggctgcc agatctgggg
     2281 catgcccagg acctcatgga tgggctgcct ccaggtgaca gcaatcagct ggcctggttt
     2341 gatactgacc tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2401 tatatatctt gtggaaagga cgaactatgg ctcgacgtgg agccgtc
//
