LOCUS       sequence-27825-e         496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCerulean-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..463
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL"
ORIGIN
        1 ggacgagctg tacaagggtt ctggaagtgg atccttggct actacacttg aacgtattga
       61 gaagaacttt gtcattactg acccaagatt gccagataat cccattatat tcgcgtccga
      121 tagtttcttg cagttgacag aatatagccg tgaagaaatt ttgggaagaa actgcaggtt
      181 tctacaaggt cctgaaactg atcgcgcgac agtgagaaaa attagagatg ccatagataa
      241 ccaaacagag gtcactgttc agctgattaa ttatacaaag agtggtaaaa agttctggaa
      301 cctctttcac ttgcagccta tgcgagatca gaagggagat gtccagtact ttattggggt
      361 tcagttggat ggaactgagc atgtccgaga tgctgccgag agagagggag tcatgctgat
      421 taagaaaact gcagaaaata ttgatgaggc ggcaaaagaa cttatcaagt gtgtggtggt
      481 gggagacgga gctgta
//
